子宫内膜腺上皮细胞凋亡及相关基因表达调节的体外研究

为探讨Ｅ２、Ｐ、ＲＵ４８６及ＴＮＦ α对子宫内膜腺体细胞凋亡及其相关基因表达的调节，离体原代培养人子宫内膜腺体细胞经 ＴＵＮＥＬ 及免疫组化法检测。结果表明：（１）外源加入Ｅ２抑制腺体细胞凋亡，刺激Ｂｃｌ ２ 的表达。（２） Ｐ 抑制腺体细胞的凋亡，不刺激Ｂｃｌ ２、Ｆａｓ 及Ｆａｓ Ｌ 的表达。（３） ＲＵ４８６ 刺激Ｆａｓ 抗原增加并诱发腺体细胞凋亡。（４） ＴＮＦ α诱发内膜腺体细胞凋亡，对Ｂｃｌ ２、Ｆａｓ 及Ｆａｓ Ｌ 的表达无影响。从而提示，Ｅ２、Ｐ及ＴＮＦα对内膜凋亡的发生均有作用； ＲＵ４８６ 抗早孕可能与其诱导凋亡的发生有关。

Study on Regulation of Endometrial Apoptosis and Its Correlated Gene Expressions in Vitro

Dept of Obstetrics and Gynecology, The Third Hospital of Beijing Medical University, Beijing 100083)ABSTRACTTo investigate regulation of endometrial apoptosis and its correlated gene expression (Bcl2, Fas, FasL) in vitro, primarily cultured endometrial glandular cells were detected apoptosis by TUNEL method. Bcl2, Fas and FasL antigens were measured by immunohistochemistry method. The results showed:(1) Apoptotic cells frequently occurred among the glandular cells after three days of culture, but it was significantly inhibited by adding estradiol or progesterone into the media, while greatly induced by RU486 or TNFα. (2) Estradiol, but not progesterone, increased Bcl2 expression in cultured glandular cells. (3) Fas antigen was sligtly stimulated only by adding RU486 into the medium. E 2, P and TNFα play roles on regulating endometrial apoptosis. The inducement of apoptosis by RU486 may be related to its contraceptive effect.