子宫内膜增生性疾病患者内膜细胞凋亡的研究

目的 :研究凋亡在子宫内膜增生性疾病中的作用。方法 :用改良原位末端标记技术检测 15例正常月经周期的增生期、分泌期、月经期子宫内膜 ,11例增殖性子宫内膜 ,12例子宫内膜癌 ,以及术前用孕激素治疗的 13例异常增生子宫内膜中的凋亡细胞 ,并计算其凋亡指数 (AI)。结果 :分泌期、月经期子宫内膜、增殖性子宫内膜、子宫内膜癌AI均比正常增生期子宫内膜AI高 (P <0 .0 1)。增殖症患者内膜不典型增生组AI比单纯增生、复杂增生组AI高 (P <0 .0 5 ) ;内膜癌患者低分化组AI比高分化组、中分化组AI高 (P <0 .0 5 )。结论 :细胞凋亡与正常子宫内膜周期性变化有关 ,而在增殖性和癌变子宫内膜中的异常表达可能与子宫内膜的良恶性病变有关

The Study of Apoptosis in Endometrial Hyperplasia and Adenocarcinoma

Objective:To investigate the role of apoptosis in endometrial hyperplasia and adenocarcarcinoma.Methods:Apoptosis was detected using in situ Tdt mediatd dUTP biotin nick ending labeling(TUNEL)in 15 normal endometrium(5 proliferative,5 secretory,5 menstrual phase),11 endometrial hyperplasia,12 adenocarcinoma and 13 abnormal endometrium treating by progesterone before operation.The apoptotic index(AI)for each case was counted.Results:AI in secretory phase,menstrual phase,endometrial hyperplasia and adenocarcinoma were significantly higher than that in normal proliferative endometrium( P <0.01).In hyperplasia,AI of atypical was significantly higher than that in simple and complex( P <0.05).In adenocarcinoma,AI of low differentiated was significantly higher than that in the other grade( P <0.05).Conclusion:Endometrial cell apoptosis is associated with cycling endometrial changes.The abnormal apoptosis may play an important role in the pathogenesis of benign and malignant changes in endometrial hyperplasia and adenocarcinoma.