Anti-apoptotic seminal vesicle protein IV inhibits cell-mediated immunity |
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Authors: | Fuggetta M P Lanzilli G Cottarelli A Ravagnan G Cartenì M De Maria S Metafora B M Metafora V Metafora S |
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Affiliation: | Institute of Neurobiology and Molecular Medicine (INMM), CNR, Area Ricerca Roma Tor Vergata, Via del Fosso del Cavaliere 100, Rome, Italy. |
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Abstract: | The in vitro effect of seminal vesicle protein IV (SV-IV) on the cytotoxic activity of human natural or acquired cellular immunity has been investigated by standard immunological procedures, a (51)Cr-release cytotoxicity assay, and labeled-ligand binding experiments. The data obtained demonstrate that: (1) fluoresceinated or (125)I]-labeled SV-IV binds specifically to the surface of human purified non-adherent mononuclear cells (NA-MNC); (2) SV-IV suppresses the cytotoxicity of natural killer (NK) cells against K562 target cells, that of IL-2-stimulated NK (LAK) cells against DAUDI target cells, and that of VEL antigen-sensitized cytotoxic T lymphocytes (CTLs) against VEL target cells; (3) treatment of K562 target cells alone with SV-IV decreases their susceptibility to NK-induced lysis. These findings indicate that the protein SV-IV has a marked in vitro inhibitory effect on NK, LAK and CTL cytotoxicity, providing a better understanding of its immune regulatory functions. |
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