NP方案加同期照射对人肺腺癌细胞存活的影响

目的 探讨NP方案加同期照射对人肺癌细胞增殖和凋亡的影响.方法 以A549、H1299为实验对象,设单纯照射组（A组）、酒石酸长春瑞滨＋顺铂＋同期照射组（B组）,再按照照射剂量不同分为A-0、A-4、A-8组和B-0、B-4、B-8组.MTT方法用于检测酒石酸长春瑞滨＋顺铂＋同期照射对细胞增殖的影响,FACS用于检测酒石酸长春瑞滨＋顺铂＋同期照射对细胞凋亡的影响和细胞表面P-gp表达的影响.结果 除B-0组在48 h细胞抑制率与A-0组比较差异无统计学意义（P=0.103）外,B-0、B-4和B-8组在6,12,24和48 h时细胞抑制率均显著低于A-0、A-4和A-8组,差异均有统计学意义（P＜0.01）.A-0组细胞凋亡率（4.01±0.95）％,A-4组为（20.19±3.76）％,A-8组为（45.34±4.77）％.A-8组细胞凋亡率显著高于A-4组,A-4组细胞凋亡率显著高于A-0组,差异均有统计学意义（t =7.17,P=0.006;t=7.23,P=0.019）.B-0组细胞凋亡率（14.78±2.37）％,B-4组为（36.18±4.73）％,B-8组为（51.12±3.37）％.B-8组细胞凋亡率显著高于B-4组,B-4组细胞凋亡率显著高于B-0组,B-0组细胞凋亡率显著高于A-0组,B-4组细胞凋亡率显著高于A-4组,差异均有统计学意义（t=3.59,P=0.037;t=9.00,P=0.003;t=7.31,P=0.018;t=4.58,P=0.020）,B-8组细胞凋亡率与A-8组比较差异无统计学意义（t=1.71,P=0.185）.A-0组S周期细胞比例为（10.61±2.23）％,A-4组为（14.75±3.02）％,A-8组为（21.81±2.94）％;B-0组S周期细胞比例为（19.27±2.97）％,B-4组为（26.23±2.12）％,B-8组为（32.37±3.53）％.B-0、B-4和B-8组S周期细胞比例分别显著高于A-0、A-4和A-8组,差异均有统计学意义（t=4.04,P=0.027;t=5.35,P=0.013;t=3.98,P=0.028）.A-0、A-4和A-8组P-gp荧光强度分别为（7.32±0.75）、（6.57±0.93）、（6.32±0.86） MFI,差异无统计学意义（P＞0.05）.B-0、B-4和B-8组P-gp荧光强度分别为（5.12±0.81）、（3.76±0.74）、（2.16±0.24） MFI.B-0组P-gp荧光强度显著低于A-0组,B-4组P-gp荧光强度显著低于A-4组,B-8组P-gp荧光强度显著低于A-8组,差异均有统计学意义（t=-3.45,P=0.041;t=-5.85,P=0.010;t=-8.07,P=0.015）.B-4组P-gp荧光强度显著低于B-0组,差异有统计学意义（t=-8.07,P=0.015）,且与B-8组比较差异无统计学意义（t=-3.56,P=0.071）.结论 NP方案加同期照射可以显著抑制人肺癌细胞增殖和凋亡,且降低肺癌细胞表面P-gp的表达.

The effect of NP regimen plus concurrent irradiation on cancer cell survival of human lung adenocarcinoma

Objective To study the effect of NP regimen plus concurrent irradiation on cancer cell survival of human lung adenocarcinoma.Methods A549 and H1299 cells were served as the experimental objects and they were divided into irradiation group （A group）,and vinorelbine tartrate injection plus cisplatin with concurrent radiotherapy group （B group）.According to the different irradiation doses A group and B group were divided into A-0,A-4,A-8 group and B-0,B-4,B-8 group.MTT was performed to measure the effect of NP regimen plus concurrent irradiation on the cell proliferation,and FACS was used to detect the effect of NP regimen plus concurrent irradiation on apoptosis and P-gp expression.Results Except the cell inhibition rate of B-0 group and A-0 group had no significant difference,the cell inhibition rate of B-0,B-4 and B-8 group at 6,12,24 and 48 h were significantly lower than those of A-0,A-4 and A-8 group.The apoptosis rate of A-8 group （45.34 ±4.77）％] was significantly higher than that of A-4 group （20.19 ± 3.76）％] （t =7.17,P =0.006）,the apoptosis rate of A-4 group was significantly higher than that of A-0 group （4.01 ± 0.95）％] （t =7.23,P=0.019）.The apoptosis rate ofB-8 group （51.12 ± 3.37）％]was significantly higher than that of B-4 group（36.18 ± 4.73）％] （t =3.59,P =0.037）,the apoptosis rate of B-4 group was significantly higher than that of B-0 group （14.78 ± 2.37）％] （t =9.00,P =0.003）.The apoptosis rate of B-0 group （14.78 ± 2.37）％] was significantly higher than that of A-0 group （4.01±0.95）％] （t =7.31,P =0.018）,the apoptosis rate of B-4 group was significantly higher than that of A-4 group （t =4.58,P=0.020）,but there was no significant difference between B-8 group and A-8 group （t =1.71,P =0.185）.The S cycle percentage of cells in B-0,B-4 and B-8 groups were significantly higher than those of A-0,A-4 and A-8 group （P ＜0.05）.There were no significant differences among the P-gp fluorescence intensity of A-0,A-4 and A-8 group （P ＞ 0.05）.The P-gp fluorescence intensity of B-0 group was significantly lower than that of A-0 group （t =-3.45,P =0.041）,the P-tp fluorescence intensity of B-4 group was significantly lower than that of A-4 group（t =-5.85,P =0.010）.The P-gp fluorescence intensity of B-8 group was significantly lower than that of A-8 group （t =-8.07,P =0.015）.The P-gp fluorescence intensity of B-4 group was significantly lower than that of B-0 group （t =-8.07,P=0.015）,and there was no significant difference between the mean fluorescence intensity of B-4 group and B-8 group （t =-3.56,P =0.071）.Conclusion NP regimen plus concurrent irradiation could significantly inhibit the proliferation and apoptosis of human lung cancer cells,and decrease the expression of P-gp in lung cancer cells.