Ghrelin和MMP-9在直肠癌组织中的表达及其与淋巴结转移的关系

目的：探讨直肠癌组织中生长激素释放肽（Ghrelin）和基质金属蛋白酶-9（MMP-9）的表达与淋巴结转移的关系。方法：应用免疫组织化学方法及Westernblot检测Ghrelin和MMP-9在21例无淋巴结转移及35例有淋巴结转移直肠癌组织中的表达水平的表达，分析Ghrelin和MMP-9与直肠癌淋巴结转移中的关系及两者的相关性。结果：Ghrelin和MMP-9在直肠癌组织中的阳性表达率分别为53．57％和80．36％，明显高于癌旁正常组织（P〈0．05）；Ghrelin和MMP-9蛋白表达与肿瘤淋巴结转移比率联系密切，并且在直肠癌组织中的阳性表达随着淋巴结转移个数增加而增加（P〈0．05）；Ghrelin与MMP-9的阳性表达呈正相关（x=0．832，P〈0．001）。结论：Ghrelin和MMP-9表达升高与直肠癌的淋巴结转移呈正相关，联合检测Ghrelin和MMP-9可能对直肠癌患者淋巴结转移及预后评估具有重要临床价值。

The Correlation between Expression of Ghrelin and MMP-9 in Rectal Cancer with Lymph Node Metastasis

Objective： To investigate the correlation between the Ghrelin and MMP-9 in rectal cancer tissue with lymph node metastasis.Method： A total of 21 cases of rectal cancer without lymphatic metastasis and 35 cases of rectal cancer with lymphatic metastasis were enrolled in this study.Immunohistochemical staining and western blot were performed to detect the expression of Ghrelin and MMP-9 in the tumor tissues of 56 patients.And statistical analysis technique was used to investigate the relation between their expression and the relationship between the expression of Ghrelin and MMP-9.Result： The expression of Ghrelin and MMP-9 in rectal cancer tissue was 53.57% and 80.36%, respectively, they were significantly highter than that in the adjacent mucosal tissues （ P〈0.05 ） .There were a close correlation between the expression of Ghrelin and MMP-9 with lymph node metastasis rate （ P〈0.05 ） .Furthermore, the positive expression of Ghrelin and MMP-9 increased with increasing lymphatic metastasls.The positive expression of Ghrelin was consistent with the positive expression of MMP-9 （r=0.832, P〈0.001 ） .Conclusion： Expressions of Ghrelin and MMP-9 are closely related to the degree of lymphatic metastasis of rectal cancer, and their combination may be an important indicator to evaluate the malignant degree and prognosis of the rectal cancer.