BMP-6改良纤维蛋白原支架软骨模块修复兔关节软骨缺损的实验研究

目的研究骨形态发生蛋白(bone morphogenic protein,BMP-6)在构建改良纤维蛋白胶支架软骨模块修复关节软骨缺损的作用。方法以软骨细胞为种子细胞种植于改良纤维蛋白胶支架上,构建细胞改良支架复合物,体外培养,并在培养系统中加入BMP-6生长因子,观察软骨细胞在支架中的繁殖情况,以及载体降解情况,并将培养出的软骨模块植入于动物关节软骨缺损模型中进行定期观测。结果改良纤维蛋白原支架可满足组织工程软骨修复需要,BMP-6生长因子用于构建模块修复关节软骨缺损模型可促进软骨细胞软骨基质分泌,并在动物试验中发现植入6周后,与对照比较在关节软骨缺损修复质量上有显著性差异。结论BMP-6生长因子作用于改良纤维蛋白胶软骨模块中可明显促进组织工程软骨模块构建以及关节软骨缺损的修复。

Study on BMP-6 on Repairing Articular Cartilage Defects by Chondrocyte-improved Fibrin Glue Bracket

Objective To study the effect of bone morphogenic protein(BMP-6)on reconstruction of improved fibrin glue bracket to repair the defection of articular cartilage.Methods The chondrocytes were seeded on improved fibrin glue bracket,cultured in vitro with BMP-6 to construct the compound.Proliferation of the cells on the bracket and degradation of bracket were observed.The compound was transplanted into the rabbit model of articular cartilage defection and observed at 6th week.Results Improved fibrin glue bracket fitted for the demand of the cartilage reparation in tissue engineering.BMP-6 accelerated the proliferation of cartilage cells on improved fibrin glue bracket.After 6 weeks when the compounds were transplanted into rabbit models,the reparation of these compounds showed significantly different from that of the control group.Conclusions BMP-6 which is applied to improved fibrin glue bracket can significantly accelerate the construction of compound and reparation of articular cartilage defection.