多溴联苯醚对SH-SY5Y细胞氧化应激及凋亡的影响

目的观察2,2’,4,4’-四溴联苯醚(PBDE-47)对人神经母细胞瘤SH-SY5Y细胞株氧化应激和凋亡的影响及活性氧在细胞凋亡中的作用。方法实验共分4组,PDDE-47终浓度为0、2、4、8μg/ml,分别作为对照组,低、中、高剂量组。用2、4、8μg/ml PBDE-47对培养的SH-SY5Y细胞染毒,24 h后检测细胞存活率、活性氧(ROS)水平、丙二醛(MDA)含量及凋亡率。结果与对照组比较,低剂量组细胞存活率略有上升,中、高剂量组细胞存活率明显降低,差异有统计学意义(P

Effcts of PBDE-47 on oxidative stress and apoptosis in SH-SY5Y cell

Objective To investigate the oxidative stress and apoptosis induced by 2, 2', 4, 4'-poly-brominated diphenyl ethers (PBDE-47) in human neuroblastoma SH-SY5Y cells, and to explore the involved role of reactive oxygen species (ROS) on apoptosis. Methods The rate of cellular survivors, intracellular ROS level, malondialdehyde (MDA) content and percentage of apoptosis were measured respectively after the SH-SY5Y cell were exposed to 2, 4, 8 |xg/ml PBDE-47 for 24 h in vitro. Results The rate of cellular survivors in the low dose PBDE-47-treaied group was higher than the control group (P<0.05), but the moderate and high dose PBDE-47-treated groups were significantly lower than the control group (P<0.05). The MDA contents in the moderate and high dose PBDE-47-treated groups were significantly higher than the control group (P<0.05) and increased with increase of PBDE-47 exposure concentrations. Compared with the control group, the levels of ROS were significantly increased with increase of PBDE-47 concentrations (P<0.05). In the moderate and high dose PBDE-47-treated groups, the percentages of apoptosis were significantly higher than that of the control group (P<0.05). Conclusion PBDE-47 can induce oxidative stress and apoptosis in SH-SY5Y cell. ROS may play an important role in the apoptosis induced by PBDE-47.