硫酸铍致人胚肺成纤维细胞氧化损伤及枸杞多糖的抗氧化效应

目的研究不同浓度的硫酸铍(BeSO4)对人胚肺成纤维细胞(MRC-5)的氧化损伤作用,并观察枸杞多糖(LBP)的保护作用。方法建立体外细胞培养实验模型,终浓度分别为1.0、10.0、100.0μmol/L的BeSO4染毒MRC-5细胞24h后,立即检测超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力及丙二醛(MDA)含量,并观察0.2mg/ml LBP对10.0μmol/L BeSO4染毒MRC-5细胞的作用。结果SOD、GSH-Px活力随BeSO4染毒剂量的增加而降低,MDA含量随染毒剂量的增加而升高,且具有一定的剂量-效应关系(rGSH-Px=-0.900,P<0.01;rSOD=0.980,P<0.01;rMDA=-0.995,P<0.01)。LBP干预组SOD、GSH-Px活力增强,MDA含量明显降低,差异有统计学意义(P<0.05或P<0.01)。结论BeSO4可致MRC-5细胞氧化产物增多,抗氧化酶活力降低,且存在剂量-效应关系,LBP可抑制BeSO4对MRC-5细胞的氧化损伤。

Study on oxidative damage of human embryonic lung fibroblasts induced by beryllium sulfate and anti-oxidation effect of lycium barbarum polysaccharides

Objective To investigate oxidative damage of human embryonic lung fibroblast(MRC-5)induced by different concentrations of beryllium sulfate(BeSO4)and observe the protective effect of Lycium barbarum polysaccharides(LBP)on MRC-5 cells.Methods Experimental model of cell culture in vitro was established,MRC-5 cells were exposed to final concentrations of 1.0,10.0,and 100.0 μmol/L of BeSO4 for 24 h.Super oxide dismutase(SOD),glutathione peroxidase(GSH-PX)activity and malondialdehyde(MDA)content were detected immediately,and the effect of 0.2 mg/ml LBP on MRC-5 cells exposed to 10.0 μmol/L BeSO4 was observed.Results SOD and GSH-PX activity reduced as the dose of BeSO4 increased,MDA content increased as the dose elevated showing a dose-response relationship(rGSH-Px=-0.900,P<0.01;rSOD=0.980,P<0.01;rMDA=-0.995,P<0.01).SOD and GSH-PX activity in the LBP intervention group increased,while MDA decreased significantly,the difference was significant(P<0.05 or P<0.01).Conclusions BeSO4 could damage MRC-5 cells causing an increase of oxidation products and a decrease of antioxidant enzyme activity and showing a dose-effect relationship.LBP could inhibit oxidative damage induced by BeSO4 on MRC-5 cells.