| 抗亚洲带绦虫药物对乳酸脱氢酶抑制作用 |
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| 引用本文： | 陈祖云,戴佳琳,黄江,申萍香,廖兴江.抗亚洲带绦虫药物对乳酸脱氢酶抑制作用[J].中国公共卫生,2010,26(5). |
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| 作者姓名： | 陈祖云 戴佳琳 黄江 申萍香 廖兴江 |
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| 作者单位： | 1. 贵阳医学院药用植物学与生药学教研室,贵阳,550004
2. 贵阳医学院多媒体形态学实验室 |
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| 基金项目： | 国家自然科学基金，贵州省科技攻关项目，贵州省农业科技攻关项目，贵州省科技攻关项目，贵州省省长基金，贵阳市科技局社发攻关项目 |
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| 摘 要： | 目的探讨抗亚洲带绦虫药物对于重组的亚洲带绦虫乳酸脱氢酶(Ta.LDH)酶促功能的影响。方法将不同浓度的吡喹酮、阿苯达唑和甲苯咪唑加入Ta.LDH所催化的丙酮酸还原成乳酸(正反应)以及乳酸氧化成丙酮酸(逆反应)的标准反应体系当中,测定烔?废汆堰?核苷酸(NADH)在A340处吸光度的变化值。采用SPSS 16.0软件进行分析,计算药物对酶活性的相对抑制率。结果与对照组比较,0.10 mmol/L吡喹酮对Ta.LDH催化正逆反应的相对抑制率分别为93.99%(P0.01)和94.67%(P0.01),0.10 mmol/L阿苯达唑分别为85.45%(P0.01)和73.81%(P0.01);0.15 mmol/L甲苯咪唑分别为92.2%(P0.01)和86.13%(P0.01)。结论Ta.LDH可能为吡喹酮、阿苯达唑和甲苯咪唑抗亚洲带绦虫药物作用的靶标分子。
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| 关 键 词： | 亚洲带绦虫 乳酸脱氢酶 重组蛋白 吡喹酮 阿苯达唑 甲苯咪唑 |
Effect of praziquantel,albendazole and mebendazole on recombinant lactate dehydrogenase of Taenia asiatica |
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| CHEN Zu-yun,DAI Jia-lin,HUANG Jiang,et al..Effect of praziquantel,albendazole and mebendazole on recombinant lactate dehydrogenase of Taenia asiatica[J].Chinese Journal of Public Health,2010,26(5). |
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| Authors: | CHEN Zu-yun DAI Jia-lin HUANG Jiang |
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| Affiliation: | CHEN Zu-yun,DAI Jia-lin,HUANG Jiang,et al.Department of Medical Plant , Pharmacognosy,School of Pharmacolgy,Guiyang Medical College(Guiyang 550004,China) |
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| Abstract: | Objective To detect the effect of praziquantel,albendazole,and mebendazole on activity of the recombinant lactate dehydrogenase of Taenia asiatica(rTaLDH).Methods The effects of praziquantel,albendazole,and mebendazole on the enzymatic activity of rTaLDH were detected by spectrophotometric method in standard reaction system established before.The solvent of each reagent was used as control.The results were analyzed with SPSS16.0 software.Results Compared with the control,considerable inhibition for both red... |
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| Keywords: | Taenia asiatica lactate dehydrogenase recombinant protein praziquantel albendazole mebendazole |
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