| 水产品中耐药大肠埃希菌整合子与耐药基因盒研究 |
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| 引用本文： | 张梦寒,沈强,朱莉勤,姜韬.水产品中耐药大肠埃希菌整合子与耐药基因盒研究[J].中国卫生检验杂志,2011(11). |
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| 作者姓名： | 张梦寒 沈强 朱莉勤 姜韬 |
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| 作者单位： | 江苏省苏州市疾病预防控制中心; |
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| 基金项目： | 苏州市“科教兴卫”青年科技项目(SWKQ0825) |
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| 摘 要： | 目的:利用水产品中分离到的耐药大肠埃希菌,研究整合子与耐药性的关系。方法:PCR扩增1类和2类整合酶及整合子,将其进行序列分析。结果:85株耐药大肠埃希菌中88%含有1类整合酶基因;其中68%能扩增此基因;1类整合子可变区PCR扩增产物谱型有17种;1类整合子最常见的基因盒有4种。还发现1株大肠埃希菌同时携带1和2两类不同的整合酶和整合子。结论:大肠埃希菌耐药性与整合子有相关性。
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| 关 键 词： | 水产品 大肠埃希菌 整合子 基因盒 |
Study on integrons and resistance gene cassettes in Escherichia coli strains with antibiotic resistance from fishery products |
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| ZHANG Meng-han,SHEN Qiang,ZHU Li-qin,JIANG Tao.Study on integrons and resistance gene cassettes in Escherichia coli strains with antibiotic resistance from fishery products[J].Chinses Journal of Health Laboratory Technology,2011(11). |
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| Authors: | ZHANG Meng-han SHEN Qiang ZHU Li-qin JIANG Tao |
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| Affiliation: | ZHANG Meng-han,SHEN Qiang,ZHU Li-qin,JIANG Tao (Suzhou Center for Disease Prevention and Control,Suzhou 215004,China) |
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| Abstract: | Objective:Escherichia coli strains with antibiotic resistance isolated from some fishery products were used to investigate the relationship between the integrons and the antibiotic resistance profile.Methods: PCR method was performed to amplify class 1 and class 2 integrases(intI1 and intI2) as well as integrons for sequencing analysis.Results: It was found that 88% of 85 E.coli strains with antibiotic resistance contained class 1 integrase gene,in which,68% can amplify this gene.There were 17 different PCR... |
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| Keywords: | Fishery products Escherichia coli Integron Gene cassette |
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