高效液相色谱法测定保健食品中阿魏酸含量的方法研究

目的:建立保健食品中阿魏酸的高效液相色谱测定方法。方法:采用WATERS Xterra RP18(4.6×250 mm,5μm)色谱柱分离;流动相为乙腈:0.5%冰醋酸=28:72(v/v);流速1.00 ml/min;2487紫外检测器322 nm测定。结果:阿魏酸在0.005μg/ml～80μg/ml时,浓度与峰面积呈良好的线形关系(r=0.9999),检出限为5.0μg/L,加标回收率在98.0%～100.8%之间,相对标准偏差RSD在1.10%～1.81%之间。结论:该方法简便、快速、准确,适用于保健食品中阿魏酸含量的测定。

Determination of Ferulic acid in health food by high performance liquid chromatography

Objective:A method has been established for the determination of Ferulic acid in health food by high performance liquid chromatography.Methods:The separation was carried out on a Xterra RP18(4.6×250 mm,5 μm) RP-column with 2487 UV detection at 322 nm,the mobile phase were acetonitrile and 0.5% acetic acid(28:72 v/v) with the flow rate at 1.0 ml/min.Results:When the ferulic was at 0.005 μg/ml~80 μg/ml,the perfect linearity between concentration and peak area ratio was obtained(r=0.9999).The detection limit was 5.0 μg/L,the recovery was 98.0%~100.8% and the RSD for the within-run precision was between 1.10%~1.81%.Conclusion:This method is simple,rapid and accurate,so that can be used for the determination of Ferulic acid in health food.