微生物改良法和电化学发光法检测血清叶酸的比较研究

目的比较微生物改良法和电化学发光法检测血清叶酸的结果,寻找两者之间规律。方法应用微生物改良法和电化学发光法对258例人群血清样品进行检测,对两种检测方法进行相关性检验和一致性分析。结果两种方法高度相关(相关系数r=0.885)。Bland-Altman法结果显示94.5%数值位于一致性界限内,Kappa检验的Kappa值为0.665,检测结果表明两种检验方法一致性一般,微生物改良法检测结果整体高于电化学发光法。结论微生物改良法与电化学发光法血清叶酸检测结果高度相关,但两种方法的一致性结果一般,用电化学发光法评价人群叶酸的营养状况可能导致叶酸缺乏人数偏高。

Comparative study of the detection of serum folate with improved microbial assay and electrochemiluminescence method

OBJECTIVE To compare the result of serum folate determined by improved microbial assay and electrochemiluminescence method,and to look for the relationship between them,so as to provide basis for the assessment of nutrition status of folate in population.METHODS A total of 258 serum samples were examined by improved microbial assay and electrochemiluminescence method.The correlation and consistence of the two method were analyzed.RESULTS The result showed that the correlation coefficient of the two method was 0.885,which indicated that the result of two method were highly correlated.Results of Bland-Altman method showed that 94.5% of the values were within the consistency limit,and the Kappa value of Kappa test was 0.665.The result of consistency analysis showed that there were some differences between the two methods,and the result of serum folate tested by improved microbial assay were higher than that of electrochemiluminescence method in general.CONCLUSION The result of serum folate tested by electrochemiluminescence were highly correlated with the improved microbial assay,yet there are some differences in the consistency result between the two methods.Evaluating the nutrition status of folate by electrochemiluminescence may lead to a higher number of folate deficiency.