miR-200a在外阴鳞癌组织中的表达及临床意义

目的:分析外阴鳞癌(vulvar squamous cell carcinoma,VSCC)组织中微小RNA-200a(miR-200a)的表达及其临床意义。方法:通过RT-PCR检测外阴鳞癌组织25例、外阴上皮内非瘤样病变组织30例(其中包括外阴增生性营养不良15例、外阴萎缩性营养不良15例)以及正常外阴组织10例中miR-200a的表达。结果:miR-200a在外阴鳞癌组织中的相对表达量为(1.162±0.421),低于外阴上皮内非瘤样病变组织(2.723±1.151)及正常外阴皮肤组织(4.341±1.507)(P<0.05),但在外阴上皮内非瘤样病变组织及正常外阴组织间表达量无明显差异。在不同临床分期、不同组织学分级的外阴鳞癌组织中miR-200a表达量有统计学差异(P<0.05)。结论:miR-200a在外阴鳞癌组织中的表达下调,提示其可能在外阴鳞癌的发生发展中起到抑癌基因的作用。

Expression and clinical significance of miR-200a in vulvar squamous cell carcinoma

Objective:To analyze the expression and clinical significance of miR-200a in vulvar squamous cell carcinoma(VSCC).Methods:The expressions of miR-200a in 25 cases with VSCC,30 cases with nonneoplastic epithelial disorders of vulve(including 15 cases with vulvar hyperplastic dystrophy and 15 cases with vulval atrophic malnutrition),and 10 cases with normal vulvar tissue were detected by RT-PCR.Results:The relative expression level of miR-200a in cases with VSCC was(1.162±0.421),which was statistically significantly lower than those in cases with nonneoplastic epithelial disorders of vulvar(2.723±1.151) and cases with normal vulvar tissue(4.341±1.507)(P<0.05),but there was no significant difference between cases with nonneoplastic epithelial disorders of vulvar and cases with normal vulvar tissue.There was statistically significant difference in miR-200a expression level among cases with VSCC of different clinical stages and histological grades(P<0.05).Conclusion:Down-regulation of miR-200a in cases with VSCC indicates that miR-200a might play a role as an anti-oncogene in pathogenesis and development of VSCC.