子宫肌瘤患者血清miR-21的表达及其与ER、PR的相关性

目的探讨子宫肌瘤患者血清miR-21表达及其与雌激素受体(ER)、孕激素受体(PR)的相关性。方法选取2016年10月-2017年10月医院收治的72例子宫肌瘤患者为研究组,选取同期于该院行健康体检的72例健康女性作为对照组。应用荧光实时定量PCR法检测两组女性血清miR-21表达,采用免疫组化染色SP法检测研究组患者子宫肌瘤组织ER、PR表达。比较研究组患者与对照组健康体检者血清miR-21表达,比较研究组患者子宫肌瘤组织ER、PR不同表达情况下血清miR-21表达,分析子宫肌瘤患者血清miR-21表达与子宫肌瘤组织ER、PR的相关性。结果研究组患者血清miR-21表达显著高于对照组,差异有统计学意义(P<0. 05)。ER阳性、PR阳性患者血清miR-21表达显著高于ER阴性、PR阴性患者,差异有统计学意义(P<0. 05)。研究组患者子宫肌瘤组织ER、PR不同表达与miR-21表达均呈正相关性,其中ER不同表达与miR-21表达呈强正相关性(P<0. 05),PR不同表达与miR-21表达呈弱正相关性(P<0. 05)。结论子宫肌瘤患者血清miR-21呈现高表达,与子宫肌瘤组织ER、PR不同表达呈正相关性,可能参与了子宫肌瘤的发生发展。

Expression of serum miR-21 and the correlations with ER,PR in patients with uterine leiomyoma

Objective To explore the expression of serum miR-21 and the correlations with estrogen receptor( ER),progesterone receptor( PR) in patients with uterine leiomyoma. Methods From October 2016 to October 2017,72 patients with uterine leiomyoma were selected as study group,and 72 healthy women undergoing physical examination during the same period were selected as control group. Fluorescence real-time quantitative PCR was used to detect the expression levels of serum miR-21 in the two groups. Immunohistochemical SP method was used to detect the expression levels of ER and PR in uterine leiomyoma of patients in study group. The expression levels of serum miR-21 in the two groups were compared. The expression levels of serum miR-21 in patients with different ER and PR expression levels in uterine leiomyoma in study group were compared. The correlations between serum miR-21 expression and ER,PR in patients with uterine leiomyoma were analyzed. Results The expression level of serum miR-21 in study group was statistically significantly higher than that in control group( P<0. 05). The expression level of serum miR-21 in patients with positive ER and PR was statistically significantly higher than that in patients with negative ER and PR( P<0. 05). In study group,ER and PR in uterine leiomyoma were positively correlated with miR-21 expression,there was a strong correlation between ER expression and miR-21 expression( P<0. 05),and there was a weak correlation between PR expression and miR-21 expression( P<0. 05). Conclusion The expression level of serum miR-21 in patients with uterine leiomyoma is high,which is positively correlated with different expressions of ER and PR,serum miR-21 may be involved in occurrence and development of uterine leiomyoma.