纳米氧化锌颗粒对大鼠肺及人支气管上皮细胞的毒性作用

目的探讨纳米氧化锌(ZnO)颗粒对大鼠肺及人支气管上皮细胞(HBE)的毒性作用。方法通过病理组织切片,观察不同质量浓度纳米ZnO滴注大鼠气管后其肺组织的病理变化。采用CCK-8法检测不同浓度纳米ZnO对HBE细胞存活率的影响,及流式细胞术检测不同浓度纳米ZnO对HBE细胞内氧化应激活性氧(ROS)、Ca2+浓度以及线粒体膜电位变化的影响。结果染毒后大鼠病理组织切片显示,随着染毒剂量的增加,大鼠肺部的炎性细胞浸润的数量逐渐增多。CCK-8法检测显示:随纳米ZnO染毒剂量的增加,HBE细胞存活率显著降低,HBE细胞内ROS及Ca2+浓度显著增加,线粒体膜电位显著降低(P0.05)。结论 (30±10)nm ZnO颗粒在整体和细胞水平时,可能通过不同机制产生不同种类的ROS,从而对细胞产生氧化损伤及应激效应。

Toxic effects of Nano-ZnO particles on rat lung tissue and human bronchial epithelial cells

Objective To study toxic effects of nano-ZnO particles on rat lung tissue and human bronchial epithelial ( HBE ) cells. Methods After treatment of different mass concentrations of ZnO drips, pathological changes of rat lung tissues were observed by pathological tissue sections. HBE cells were treated by different concentration of nano-ZnO particles, survival rate was analyzed by CCK-8 kit;while changes of reactive oxygen species( ROS) , Ca2+ concentration, mitochondria membrane potential ( MMP) were de-termined by flow cytometry. Results After treated by ZnO,pathological tissue section analysis showed number of inflammatory cells infiltration in rat lung increased gradually with dose incensement . CCK-8 analysis shoawed HBE cell survival rate significantly de-creased with dose incensement ROS and Ca2+ concentration increased significantly,MMP decreased significantly(P<0. 05). Conclu-sion (30 ± 10) nm nano-ZnO particles could induce toxic reaction in animals and HBE cells by producing different kind of ROS by various mechanisms to make oxidative damage and stress effects on cells.