肝细胞癌组织和癌周组织中p53基因密码子249突变的比较研究

目的：本研究通过比较研究ＨＣＣ 癌组织和癌周组织中ｐ５３ 基因密码子２４９ 特征性突变来进一步阐明该基因在ＨＣＣ 发生机制中的意义。方法：收集９ 份癌组织中经单链构型多态性分析研究证实存在密码子２４９ 突变的ＨＣＣ 标本和１１ 例无此突变的ＨＣＣ 标本，采用等位基因特异性ＰＣＲ（ＡＳ－ ＰＣＲ） 方法比较研究癌组织和癌周组织中密码子２４９ 突变情况。结果：２０ 例ＨＣＣ 中，癌周组织中密码子２４９ 特征性突变检出率为７０ ％ 。９ 例ＳＳＣＰ 阳性者的癌周组织中的特征性突变检出率达１００ ％ 。１１ 例阴性患者的癌组织和癌周组织也能检出一定水平的突变。结论：ｐ５３基因密码子２４９ 突变在非癌肝组织中广泛存在。癌组织中密码了２４９ 特征性突变可能发生在肿瘤形成之前。

Comparison of codon 249 mutations of p53 gene in cancerous tissues with that in pericancerous tissues of HCCs

Objective:To analyze codon 249 mutation of p53 gene in cancerous tissue and pericancerous tissue of HCC comparatively,and study the significance of p53 gene in hepatocarcinogenesis.Methods:Codon 249 mutations were detected using allele specific PCR in cancerous and pericancerous tissues of 9 codon 249 mutation positive cases and 11 negative cases detected by single stranded conformational polymorphism (SSCP) analysis in former research.Results:The detective rate of codon 249 mutation in pericancerous tissues was 70%(14/20).That in 9 cases of SSCP positive HCCs was 100%(9/9).Codon 249 mutations were also detected in cancerous and pericancerous tissues of 11 SSCP negative HCCs.Conclusions:Codon 249 mutations of p53 gene were very popular in non neoplastic tissues.Those mutations in cancerous tissues might take place in the stage before the formation of tumor.