鼻咽癌放射敏感性异质性的形态学差异观察

目的探讨鼻咽癌放射敏感性异质性与形态学变化的相关性。方法应用电镜观察并用体视学技术测算,比较鼻咽癌细胞系CNE-2Z亚克隆株H5和S1,于体内体外照射后形态学改变的差异;用流式细胞仪、荧光显微镜检测H5和S1放射后凋亡率的不同,用Western-blot检测H5和S1放射后凋亡蛋白Bcl-2表达的差异,以期探讨其形态学异质性机制。结果H5和S1照射后形态学改变差异有显著性;照射后的H5比S1凋亡率高,对射线敏感;S1细胞的各个照射剂量组Bcl-2蛋白表达无明显差异;而H5细胞的各个照射剂量组Bcl-2蛋白表达随照射剂量的增加而递减。结论H5、S1照射后形态学的差异变化可用于预测其放射敏感性的异质性。

Morphological difference and radiosensitive heterogeneity in nasopharyngeal cancer

Objective To study the correlation of radiosensitive heterogeneity with morphological differences in nasopharyngeal cancer(NPC).Methods The subtypes H5 and S1 of NPC cell line CNE-2Z were irradiated in vivo and in vitro.The morphological changes before and after irradiation were observed with electron microscope and stereological measurement;the apoptosis-inducing capacity of H5 and S1 was detected by FCM and fluorescence microscope;Bcl-2 protein expression of H5 and S1 cells was detected by Western-blot.Results The morphological changes in H5 and S1 after irradiation had statistical difference;H5 had a higher apoptosis rate and was more radiosensitive than S1.Conclusion Changes in the morphological difference between H5 and S1 after irradiation can be used to predict their radiosensitive heterogeneity.