邻苯二甲酸二辛酯亚慢性暴露对SD大鼠血细胞的影响

目的：探讨邻苯二甲酸二辛酯（DEHP）对大鼠血细胞的影响。方法：SD大鼠设对照组（饲喂基础饲料）、4个DEHP染毒组（分别为5、50、500和2 500 mg/kg），每组20只，雌雄各半，连续饲喂染毒90 d。染毒结束后，取大鼠全血测定各类血细胞的变化。结果：与对照组相比，白细胞参数中，2 500 mg/kg组雄性大鼠嗜酸性粒细胞显著减少（P < 0.01），雌性大鼠白细胞总数显著降低（P < 0.05）；500和2 500 mg/kg组雌性大鼠淋巴细胞显著增加（P < 0.05）。红细胞参数中，2 500 mg/kg组雄性大鼠红细胞总数、血红蛋白（HGB）和红细胞压积（HCT）显著下降（P < 0.01）；雌性大鼠500和2 500 mg/kg组血红蛋白（HGB）显著下降（P < 0.05）；500和2 500 mg/kg组的平均血红蛋白含量（MCH）显著下降（P < 0.05或P < 0.01）；2 500 mg/kg组的平均血红蛋白浓度（MCHC）和直接测定的血红蛋白浓度（CHCM）显著下降（P < 0.05或P < 0.01）。未发现DEHP对雌雄大鼠血小板各指标产生影响。结论：一定剂量条件下，DEHP可对大鼠白细胞和红细胞相关指标产生影响。

Toxicity to blood cells of SD rats from sub-chronic exposure to DEHP

OBJECTIVE: To observe toxicological effects in blood cells in rats after treatment with different doses (0,5,50,500,2 500 mg/kg) DEHP. METHODS: Rats were divided into control and four treatment groups. The treatment groups were exposed to four concentrations of DEHP (5,50,500 and 2 500 mg/kg) for 90 days. At the end of the exposure,whole blood samples were taken to measure the changes of various types of blood cells. RESULTS: Compared with the control group,the leukocyte parameters for the 2 500 mg/kg dose groups were:eosinophils decreased significantly (P < 0.01),the total number of white rats significantly decreased (P < 0.05). Lymphocytes in 500 and 2 500 mg/kg groups were significantly increased (P < 0.05). In the erythrocyte parameters,the total number of red blood cells,hemoglobin (HGB) and hematocrit (HCT) in the 2 500 mg/kg group were significantly decreased (P < 0.01) while the levels of hemoglobin (HGB) in 500 and 2 500 mg (P < 0.05 or P < 0.05). Mean hemoglobin content (MCH) in 500 and 2 500 mg/kg groups decreased significantly (P < 0.05 or P < 0.01). The measured hemoglobin concentration (CHCM) decreased significantly (P < 0.05 or P < 0.01). On the other hand,DEHP had little effect on platelet parameters. CONCLUSION: DEHP exposure affected blood cells of rats under certain dosages.