枸杞多糖对酒精性肝损伤小鼠肾脏的保护作用

目的:研究枸杞多糖对酒精性肝损伤小鼠肾脏的保护作用。方法:将60只小鼠随机分为正常对照组,模型组和枸杞多糖低、中、高剂量组(分别为75、150、300 mg/kg)。枸杞多糖组动物连续灌胃受试物16 d,第10天开始,给予枸杞多糖组和模型组50%酒精连续灌胃7 d,建立酒精性肝损伤模型。最后一次灌胃16 h后处死小鼠,取血清和肾脏。用自动生化分析仪检测血清中葡萄糖(GLU)、尿素氮(BUN)和肌酐(CREA)浓度,用生化试剂盒测定肾脏中超氧化物歧化酶(SOD)、谷胱甘肽(GSH)和丙二醛(MDA)浓度,用ELISA方法测定肾脏中肿瘤杀伤因子-α(TNF-α)、白细胞介素-1β(IL-1β)浓度。结果:与对照组相比,模型组小鼠体质量下降,肾脏指数均升高,血清GLU、BUN、CREA浓度升高,肾脏SOD活性、GSH浓度下降,而MDA上升,肾脏炎性因子TNF-α和IL-1β升高(P均<0.01),表明模型组小鼠在大量酒精刺激后,机体健康受到影响,肾脏受到损伤。与模型组相比,枸杞多糖中剂量组小鼠体质量增加,各剂量组肾脏指数均降低,中、高剂量组小鼠血清GLU浓度(7.63、7.82 mmol/L)降低,高剂量组BUN浓度和CREA浓度降低,而SOD活力升高,各剂量组GSH浓度升高,MDA浓度下降,各剂量组TNF-α浓度降低(P<0.05),高剂量组IL-β浓度与模型组相比降低(P均<0.05)。结论:枸杞多糖对于乙醇诱导的小鼠酒精性肾脏损伤具有一定的保护作用。

Protective effects of lycium barbarum polysaccharide on kidney in alcohol-induced liver injury in mice

OBJECTIVE:To investigate the kidney protective effects of lycium barbarum polysaccharide (LBP) in alcohol-induced liver injury in mice.METHODS:60 male mice were randomly divided into five groups,control,model and treatment with different concentrations of LBP (75,150,300 mg/kg) groups.Alcoholic liver injury model was induced by 50% ethanol for 7 days.At 16 h after the last treatment,serum and kidney samples were obtained for the determination of biochemical markers.The concentrations of GLU,BUN and CREA in serum were determined by automatic biochemical analyzer,and the levels of superoxide dismutase (SOD),glutathione (GSH),malondialdehyde (MDA) and inflammatory factor TNF-α,IL-1β in kidneys were measured by commercial kits.RESULTS:Compared with the control group,the weights of mice in the model group were decreased significantly (P < 0.01) but,the kidney indexes were increased significantly (P < 0.01),indicating that alcohol caused organ damage in mice.Compared with the model group,the average weight of middle dose group was increased significantly (P < 0.05),and the kidney indexes for the three dose groups were decreased significantly (P < 0.05).The contents of GLU in the medium and high dose groups (7.63、7.82 mmol/L) were decreased significantly (P < 0.05).In addition,the contents of BUN and CREA in high dose group were significantly decreased (P < 0.05),the activities of SOD and contents of GSH were increased significantly (P < 0.01),and the contents of MDA were decreased significantly (P < 0.05) in all of treatment groups.The contents of TNF-α were reduced significantly (P < 0.05),the contents of IL-1β were reduced significantly (P < 0.05) only in high dose group compared with model group.CONCLUSION:Our study demonstrates that lycium barbarum polysaccharide have kidney protective effect on alcohol-induced alcoholic liver injury.