甘草干姜汤抗小鼠乳腺癌实验研究

目的：探讨甘草干姜汤(LDGD)对荷乳腺癌小鼠肿瘤生长、免疫器官及肝肾功能的影响。方法：雌性BALB/c小鼠随机分为5组：正常对照组，模型对照组，LDGD低、中、高剂量组(分别为0.1、0.3、0.9g/kg)。正常对照组小鼠灌胃给予蒸馏水，其他各组小鼠接种乳腺癌4T1细胞后，次日开始LDGD低、中、高剂量组小鼠连续灌胃给药20d，模型对照组小鼠给予相应体积的蒸馏水。测定各组小鼠的体质量、胸腺系数、脾脏系数、肝脏系数、肾脏系数、血清肝功能、肾功能生化指标及肿瘤体积、质量，HE染色后观察荷瘤小鼠的肿瘤组织病理变化。结果：与模型对照组相比，LDGD高剂量组小鼠肿瘤生长受到明显抑制，抑瘤率为(44.76±0.06)%(P<0.01)，中剂量组抑瘤率为(15.68±0.21)%(P<0.05)，低剂量组无明显抑瘤作用；肿瘤组织病理切片HE染色结果表明，LDGD高剂量组中肿瘤坏死灶区域显著增多；各剂量组LDGD对小鼠体质量均无明显影响，不同剂量LDGD组的胸腺系数、肝脏系数、肾脏系数均无明显变化，脾脏系数下降；LDGD高剂量组ALP和CRE与正常对照组小鼠的差异有统计学意义(P<0.05)，但与模型对照组相比无明显差异，其他肝肾血生化指标与正常对照组小鼠比较差异均无统计学意义。结论：高剂量LDGD对4T1荷瘤小鼠的肿瘤生长具有明显的抑制作用，无明显免疫抑制作用和肝肾毒性。

Effectiveness of licorice and dried ginger decoction against mouse breast cancers

OBJECTIVE：To investigate effects of licorice and dried ginger decoction (LDGD) on tumor growth， immune organs， and liver and kidney functions in mice with breast cancers. METHODS： Female BALB/c mice were randomly divided into 5 groups：normal control group，model control group，and LDGD low-，medium-，and high-dose groups (0.1，0.3，and 0.9 g/kg，respectively). The mice in the normal control group were given distilled water by gavage. After the mice in other groups were inoculated with breast cancer 4T1 cells，the mice in the three LDGD groups were given continuous intragastric administration for 20 days from the next day， and the mice in the model control group were given corresponding volume of distilled water. The body weight，thymus index，spleen index，liver index，kidney index，serum liver function，renal function biochemical indexes， tumor volume and mass of mice in each group were measured. Pathological changes in tumor tissues from tumor-bearing mice were observed after HE staining. RESULTS：Compared with the model control group， tumor growth of 4T1 transplanted tumor mice in LDGD high-dose group was significantly inhibited， the tumor inhibition rate was (44.76 ±0.06)% (P<0.01)， the tumor inhibition rate in LDGD medium-dose group was (15.68±0.21)% (P<0.05)，and there was no obvious tumor inhibition effect in LDGD low-dose group. HE staining results of tumor histopathological sections showed that，compared with the model control group，tumor necrosis areas in LDGD high-dose group were significantly increased. In addition， LDGD at different doses had no significant effect on the body weight of mice，and indices of thymus，liver and kidney did not change significantly，but the spleen indices decreased. There were significant differences in ALP and CRE between LDGD high-dose group and normal mice，but no significant differences between LDGD high- dose group and model control group， and no significant differences in other liver， kidney and blood biochemical indices between LDGD high-dose group and normal mice. CONCLUSION：High dose LDGD had a significant inhibitory effect on tumor growth in 4T1 tumor-bearing mice，without obvious immunosuppression and hepatorenal toxicity.