舒林酸对胃癌细胞生长抑制作用的体外观察

背景与目的:体外研究舒林酸对人胃癌BGC-823细胞的生长抑制作用,探讨其作用机制。材料与方法:将舒林酸作用于人胃癌BGC-823细胞,并设置不同的作用浓度和作用时间。以体外药物敏感实验(单核细胞直接细胞毒测定Mono-nuclearcelldireccytotoxicityassay,MTT)检测舒林酸在不同浓度及不同作用时间下对人胃癌BGC-823细胞的增殖抑制效应;流式细胞仪检测胃癌细胞周期分布;透射电镜观察药物作用后细胞凋亡的形态学改变;免疫组化检测细胞增殖(ki-67)、凋亡抑制基因(bcl-2)及还氧合酶(COX-2)蛋白的表达。结果:舒林酸对人胃癌BGC-823细胞有生长抑制作用,使G0/G1期比例增高,S期比例降低;透射电镜观察到细胞凋亡的形态特征及凋亡小体,而ki-67、bcl-2及COX-2蛋白表达阳性率显著降低;上述作用均呈时间和剂量依赖性。结论:舒林酸在体外具有良好的抑制胃癌BGC-823细胞生长的作用,其机制涉及影响细胞周期分布、诱导细胞凋亡及抑制COX-2、ki-67及bcl-2蛋白的表达。

Growth Inhibition of Sulindac on Human Gastric Cancer Cells

BACKGROUND&AIM: To study the effect of sulindac on growth inhibition of human gastric cancer cells BGC_823in vitro and its antineoplastic mechanisms. MATERIAL AND METHODS: Human gastric cancer cells BGC_823were incubated with various concentrations of sulindac for different periods.Antiproliferation effects were measured by MTT colorimetric assay,flow cytometry was used for cell cycle distribution,transmission electron microscopy was used to examine cell apoptosis morphology,immunohistochemical staining was used to measure the expressions of ki_67,bcl_2and COX_2in cells. RESULTS: Sulindac could inhibit the growth of BGC_823cells,increased the proportion of cells in the G 0 /G 1 phase,and decreased the proportion of cells in the S phase.Some morphologic features of apoptosis and the apoptosis bodies were examined by transmission electron microscopy,showing a decreased expressions of ki_67,bcl_2and COX_2in cells.All the effects demonstrated a time_and dose_dependent relationship. CONCLUSION: Sulindac could significantly inhibit the growth of human gastric cancer cells BGC_823in vitro and the antitumor mechanism may be related to affecting cell cycle distribution,inducing cell apoptosis and inhibiting the expression of COX_2,bcl_2,and ki_67.