Notch信号通路在肝癌细胞迁移过程中的作用及机制

目的:探讨Notch信号通路在肝癌细胞迁移过程中的作用及相关机制。方法:体外培养肝癌细胞系SMMC-7721、MHCC97H和正常非肿瘤肝细胞系HL-7702，利用Transwell小室检测细胞的迁移能力，Western blot检测Notch1、Snail、E-cadherin、COX-2(环氧化酶-2)蛋白的表达。分别采用5μmol/L DAPT（3，5-二氟苯乙酰-L-丙氨酰-S苯基甘氨酸t-丁酯，γ-分泌酶抑制剂）阻断Notch信号通路和50μmol/L NS-398阻断COX-2，比较对细胞迁移能力的影响以及对肝癌细胞中Snail、E-cadherin表达的影响。结果:Notch1、Snail、E-cadherin、COX-2参与了肝癌细胞迁移过程，DAPT通过阻断Notch显著上调E-cadherin，影响细胞的迁移（P<0.05），下调Snail和COX-2，NS-398通过阻断COX-2影响细胞的迁移（P<0.05）。结论:Notch信号通路在肝癌细胞迁移过程中起着非常重要的作用，其机制初步认为与Snail、E-cadherin、COX-2的表达有关。

The mechanism of Notch signaling pathway in the migration of hepatocellular carcinoma cells

Objective:To explore the mechanism of Notch signaling pathway in the migration of hepatocellular carcinoma cells.Methods:Cultured human hepatoma SMMC-7721,MHCC97H and normal liver HL-7702 cells in vitro,using transwell assay to test the ability of cell migration.Using Western blot to detect the protein expression of Notch1,Snail,E-cadherin and COX-2.Compared the cell migration ability before and after blocking Notch signaling pathway with 5μmol/L DAPT (γ secretase inhibitors） and blocking COX-2 with 50μmol/L NS-398.Results:Notch1,Snail,E-cadherin and COX-2 were involved in the migration process of HCC cells.The effect of DAPT could up-regulate the expression of E-cadherin and down-regulate the expression of Snail and COX-2.Conclusion:The role of Notch signaling pathway in HCC cells migration is very important，and the mechanism maybe related to the levels of Snail,E-cadherin and COX-2.