小檗胺对胃癌细胞增殖、凋亡的影响及其分子机制

目的:探讨小檗胺对胃癌细胞（AGS和SGC-7901）增殖、凋亡的影响并探讨其分子机制。方法:利用MTT 实验检测胃癌细胞的增殖，利用集落形成实验检测胃癌细胞的生长， 流式细胞技术检测胃癌细胞的凋亡率以及细胞周期， Western blot检测凋亡相关蛋白的表达水平。结果:小檗胺（0~64 μg/ml）能够剂量依赖性以及时间依赖性的抑制 AGS和SGC-7901细胞的增殖 （P＜0.05）。集落形成实验结果显示小檗胺(32 μg/ml)能够抑制AGS和SGC-7901细胞的生长 （P＜0.05）。流式细胞实验显示，小檗胺(32 μg/ml)同时能够促进AGS和SGC-7901细胞的凋亡 （P＜0.05），增殖G0/G1期细胞比例，降低S期细胞比例 （P＜0.05）。Western blot实验结果显示小檗胺(32 μg/ml)能够增加AGS和SGC-7901细胞的cleaved caspase-3,cleaved caspase-9以及Bax的蛋白水平，同时降低Bcl-2的蛋白水平 （P＜0.05）。结论:小檗胺能够抑制胃癌细胞的增殖，其机制可能与改变细胞周期以及促进细胞凋亡有关。

Effects of berbamine on cell proliferation and apoptosis in gastric cancer cells and its molecular mechanism

Objective:To investigate the effects of berbamine on the cell proliferation in gastric caner cell lines (AGS and SGC-7901 cells),to explore its underlying mechanisms.Methods:Cell proliferation was determined by MTT assay.Cell growth was determined by colony formation assay.Flow cytometry was used to determine cell apoptosis and cell cycle,Western blot was used to determine apoptosis-related protein levels.Results:Berbamine (0~64 μg/ml) suppressed AGS and SGC-7901 cell proliferation in a concentration- and time-dependent manner (P＜0.05).Colony formation assay showed that berbamine (32 μg/ml) suppressed AGS and SGC-7901 cell growth (P＜0.05).Flow cytometry analysis showed that berbamine (32 μg/ml) induced apoptosis,and increased cell population at G0/G1 phase and decreased the cell population at S phase in AGS and SGC-7901 cells (P＜0.05).Western blot results showed that berbamine (32 μg/ml) increased the protein levels of cleaved caspase-3,cleaved caspase-9 and Bax,and decreased the protein level of Bcl-2 in AGS and SGC-7901 cells (P＜0.05).Conclusion:Berbamine suppressed cell proliferation in gastric cancer cells,possibly via inducing apoptosis and modulating cell cycle.