B7-H4在宫颈癌组织中的表达及与临床病理特征的关系

目的:检测免疫共刺激分子B7-H4在不同宫颈组织中的表达,研究其异常表达与宫颈癌患者临床病理特征的关系。方法:选择陕西省肿瘤医院病理科2014年至2018年的石蜡包埋标本162例,采用免疫组织化学方法检测20例正常宫颈、30例宫颈上皮内瘤变(CIN)和112例Ⅰ期-Ⅳ期宫颈癌组织中B7-H4的表达水平。结果:正常宫颈组织、CIN组织、宫颈癌组织中B7-H4的阳性表达率分别为0%、20.00%、47.32%,两两相比,具有显著性差异(P<0.05)。在宫颈癌组织中,B7-H4的表达与患者年龄、病理分级无明显相关性,而随着临床期别的升高、肿瘤体积的增大、浸润深度的加深、脉管瘤栓及淋巴结转移的发生,宫颈癌组织中B7-H4的阳性表达率升高。化疗前宫颈癌组织中B7-H4阳性表达率明显高于化疗后(P<0.05)。结论:负性免疫共刺激分子B7-H4在宫颈癌的发生、进展中起到了重要作用,其作用可能为宫颈癌细胞逃逸机体免疫的机制之一。检测或干预宫颈癌组织中的B7-H4表达,可能对预测宫颈癌的发生、发展及免疫治疗具有重要意义。

Expression of B7-H4 in cervical cancer tissue and its relationship with clinicopathological features

Objective:To detecte the expression of immune costimulatory molecule B7-H4 in different cervical tissues,and study the relationship between its abnormal expression and clinicopathological features of cervical cancer patients.Methods:Using immunohistochemical SP method to detecte the expression of B7-H4 in different tissues in pathology department of Shaanxi Provincial Cancer Hospital with 162 paraffin-embedded specimens from 2014 to 2018 including 20 cases of normal cervical tissues,30 cases of cervical intraepithelial neoplasia(CIN)tissues and 112 cases of cervical cancer tissues with stageⅠ-Ⅳ.Results:The positive rate of B7-H4 on normal cervix,CIN and cervical cancer tissue was 0%,20.00%,47.32%respectively,and the differences between groups had statistical significance(P<0.05).In cervical cancer tissues,the expression of B7-H4 had no obvious relationship with the patient age and pathologic grade.With the increase of clinical stage and tumor size,infiltration depth deepening,the vascular tumors and lymph node metastasis,the expression of B7-H4 of cervical cancer increased.The positive expression rate of B7-H4 in cervical cancer tissue before chemotherapy was higher than that after chemotherapy(P<0.05).Conclusion:Negative immune stimulating molecules B7-H4 played important roles in the occurrence and progress of cervical cancer.The synergy may be one of the mechanisms for cervical cancer cells escape the body's immune system.Detection or intervention of B7-H4 expression in cervical cancer may be of great significance in predicting the occurrence,development and immunotherapy of cervical cancer.