miR-34c-3p与M2型肿瘤相关巨噬细胞在三阴性乳腺癌中的表达及意义

目的:检测miR-34c-3p与M2型肿瘤相关巨噬细胞（tumor-associated macrophage,TAM)在三阴性乳腺癌(triple negative breast cancer,TNBC)中的表达，并探讨其在乳腺癌发病中的意义。方法:选择2017年2月至2018年1月于西京医院就诊的68例TNBC患者作为实验组(A组)，以同期就诊的70例乳腺纤维腺瘤患者作为对照组(B组)。采用Real-time RT-PCR 检测组织标本中miR-34c-3p的表达；流式细胞检测M2型TAM的表达；ELISA法检测血清中IL-10的表达。并进一步分析miR-34c-3p的表达与乳腺癌M2型TAM及血清IL-10水平的相关性。结果:A、B组miR-34c-3p的相对表达量分别为0.84±0.08、1.29±0.71，A组明显低于B组，差异有统计学意义(P<0.05)。与B组比较，A组M2型TAM(CD68+CD163+)细胞比例显著升高(P<0.05)。A组血清IL-10的表达［(19.69±4.93) pg/ml］较B组［(17.26±3.51) pg/ml］显著升高，差异有统计学意义(P<0.05)。TNBC组织中miR-34c-3p的表达与M2型TAM比例及血清IL-10表达均呈显著负相关(r=-0.508，r=-0.656，P<0.05)。结论:TNBC组织中miR-34c-3p的表达下调，M2型TAM比例及血清IL-10表达均显著升高，促进TNBC进展。

Expression and significance of miR-34c-3p and M2 type of tumor associated macrophage in triple negative breast cancer

Objective:To detect the expression of miR-34c-3p and M2 type of tumor-associated macrophage (TAM) in triple negative breast cancer (TNBC) patients and explore their significance in the pathogenesis of breast cancer.Methods:All 68 TNBC patients in Xijing Hospital from February 2017 to January 2018 were selected as the experimental group (group A)，and 70 breast fibroadenoma patients in the same period were treated as the control group (group B).Real-time RT-PCR was used to detect the expression of miR-34c-3p.M2 type of TAM expression was detected by flow cytometry.The expression of IL-10 in serum was detected by ELISA.To analyze of the correlation between miR-34c-3p and M2 type of TAM and IL-10.Results:miR-34c-3p in group A was significantly lower than that in group B，and the relative expression level was 0.84±0.08，1.29±0.71 respectively，and the difference was statistically significant (P<0.05).Compared with group B，the proportion of M2 type of TAM in group A (CD68+CD163+) was increased significantly (P<0.05).The expression of IL-10 in group A ［(19.69±4.93) pg/ml］ was significantly higher than that in group B ［(17.26±3.51) pg/ml］（P<0.05）.The expression of miR-34c-3p in TNBC tissues was negatively correlated with the ratio of M2 type of TAM and serum IL-10 level (r=-0.508，r=-0.656，P<0.05).Conclusion:The expression of miR-34c-3p in TNBC tissues was down-regulated，and the proportion of M2 type of TAM and serum IL-10 expression were significantly elevated，which contributed to the progression of TNBC.