乳腺癌复发转移与基因表达的相关性研究

目的:研究BCL2L2、CCNA1、CD44、CTSD和MAP2K7基因表达与乳腺癌复发转移特性的关系.方法:81例乳腺癌患者术后5年随访期内出现复发者为研究组(41例)和未复发仍存活者为对照组(40例).提取所有病例石蜡包埋组织的RNA,质量鉴定后,应用实时定量RT-PCR芯片技术平行检测BCL2L2、CCNA1、CD44、CTSD和MAP2K7基因的表达,分析各基因表达差异与乳腺癌复发转移之间的关系.结果:CCNA1、CD44和MAP2K7基因在复发病例中均高表达(57.5%、66.7%和58.5%),而BCL2L2和CTSD基因在复发组中低表达(56.1%和53.7%),BCL2L2、CCNA1、CD44、CTSD和MAP2K7基因表达水平在复发与未复发组间差异均有统计学意义,P均<0.05.BCL2L2和CTSD基因是乳腺癌复发转移的危险因素.CCNA1、CD44和MAP2K7基因对乳腺癌复发转移是保护因素.结论:实时定量RT-PCR芯片技术能快速、准确地检测肿瘤相关特性基因AR、CCND1和ESR2的表达.乳腺癌术后BCL2L2、CCNA1、CD44、CTSD和MAP2K7基因的差异表达分析可做为预防和控制该病复发的新线索.

Expression levels of genes associated with postoperative recurrence of breast cancer patients

OBJECTIVE:To detect the expression profile of BCL2L2,CCNA1,CD44,CTSD and MAP2K7 genes related to postoperative recurrence of breast cancer,and discuss their relationship with the disease progression.METHODS:There were 81 patients with breast cancer enrolled in this study.The study group included 41 cases with recurrence during 5 years follow-up,and the control group included 40 cases without recurrence.The total RNAs were extracted and identified from the paraffin-embedded tissues of all the cases.BCL2L2,CC...