人工蛹虫草子实体对Leiws肺癌荷瘤小鼠CD4^+CD25^+调节性T细胞的影响 |
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引用本文: | 樊慧婷,林洪生,李杰,祁鑫,裴迎霞,吴皓.人工蛹虫草子实体对Leiws肺癌荷瘤小鼠CD4^+CD25^+调节性T细胞的影响[J].齐鲁肿瘤杂志,2009(15):1130-1134. |
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作者姓名: | 樊慧婷 林洪生 李杰 祁鑫 裴迎霞 吴皓 |
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作者单位: | 中国中医科学院广安门医院肿瘤科,北京100053 |
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基金项目: | 科技部国际合作项目(2006DFA31700) |
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摘 要: | 目的:观察人工蛹虫草子实体(CCM)对Lewis肺癌移植瘤生长与转移的作用,同时检测CCM对荷瘤鼠CD4^+ CD25^+调节性T细胞的影响,探讨其治疗肿瘤的免疫调节机制。方法:建立Lewis肺癌移植肿瘤模型。给予CCM处理后,观测肿瘤体积动态变化,计数转移灶数目,计算抑瘤率;采用流式细胞术分别于肿瘤生长第6、11、16和21d检测荷瘤鼠脾细胞CD4^+ CD25^+的比例;荧光定量RT—PCR检测肺转移灶组织Foxp3和TGF-β mRNA的表达水平。结果:CCM可抑制Lewis肺癌移植肿瘤的生长与转移。肿瘤生长的早期这种抑制作用比较明显,在肿瘤生长21d抑制率虽为28.6%,但治疗组肺转移灶数目明显降低,P〈0.01。与模型组相比,CCM治疗组CD4^+CD25^+ T细胞增高缓慢,在肿瘤生长21d时,模型组脾脏CD4^+ CD25^+T细胞比例为17.0%,而CCM组为13.5%,两者差异有统计学意义,P〈0.01。CCM纽肺转移灶组织Foxp3和TGF-β mRNA的表达也明显低于模型组。结论:CCM抑制肿瘤生长和转移作用可能与CCM下调荷瘤体内CD4^+ CD25^+ T细胞,抑制TGF-β分泌,从而激发机体的抗肿瘤免疫相关。
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关 键 词: | 冬虫夏草/药理学 免疫/药物疗法 肿瘤 实验性 中药疗法 小鼠 |
Effect of cultured cordyceps militaris on CD4^+ CD25^+ regulatory T cells in Lewis lung carcinoma bearing mice |
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Authors: | FAN Hui ting LIN Hong-sheng LI Jie QI Xin PEI Ying-xia WU Hao |
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Affiliation: | (Department of Oncology,Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, P.R. China) |
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Abstract: | OBJECTIVE: To study the inhibitory effect of cultured cordyceps militaris(CCM)on growth and metastasis of Lewis lung carcinoma and potential relationship with CD4^+ CD25^+ regulatory T cells. METHODS: The models were established by injected Lewis lung cancer cells subcutaneously to the right axilla of C57BL/ 6 mice. The dynamic change of tumor volume, tumor weight, and number of tumor colonies in lung were observed afler treated with CCM. The changes of percentage of CD4^+ CD25^+ regulatory T cells were detected at various time d6, d11 , d16 , d21 by flow cytometry (FCM) and the mRNA expression of Foxp3 and TGF-β in I.ung metastasis were analyzed by quantitative RT PCR after treatment with CCM. RESULTS: CCM could inhibit growth and metastasis of Lewis lung cancer, especially at early stage of tumor growth. Although the inhibitory rate was(28.6%)at advanced stage, the number of tumor colonies in lung in CCM group was lower than that of control group(P〈0. 01). Compared with control group, the percentage of CD4^+ CD25^+ regulatory T cells in CCM treatment group increased slowly. The average percentage of CD4^+ CD25^+ regulatory T cells from spleen of CCM and control group were 13.5% and 17.0% respectively at d21 (P〈0. 01). The mRNA expression of Foxp3 and TGF-β in I.ung metastasis was also lower than control group. CONCLUSIONS: CCM can inhibit growth and metastasis of Lewis lung carcinoma. This effect partially is due to decreasing CD4^+ CD25^+ regulatory T cells and inhibiting TGF-β secretion, thus restore the anticancer immune response. |
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Keywords: | cordyceps sines/pharmacology immunity/drug therapy neoplasms exprimental/drug therapy(TCD) mice |
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