哈萨克族食管癌组织中microRNA-21的表达及其意义

目的：探讨哈萨克族食管癌组织中microRNA-21（miRNA-21）的表达及其意义。方法收集72例哈萨克族食管癌患者临床样本，采用实时定量PCR检测食管癌组织、周围正常食管组织miRNA-21表达水平；以患者性别、年龄、临床分期、肿瘤部位、分化程度、淋巴结转移、病情是否恶化等作为观察指标，分析miRNA-21与临床资料的关系。再以肿瘤中位生存期作为观察指标，筛选出影响哈萨克族食管癌患者预后的危险因素。结果食管癌组织miRNA-21相对表达量为（7.57±0.14），周围正常食管组织相对表达量为（1.37±0.08），食管癌组织相对表达量高于正常组织（P﹤0.05）。72例食管癌患者分为miRNA-21高表达组42例和低表达组30例，miRNA-21表达与临床分期、淋巴结转移、肿瘤进展有关，即淋巴结转移、肿瘤进展及肿瘤临床分期越晚，肿瘤miRNA-21表达也越高（P﹤0.05）；miRNA-21高表达组5年生存率为20.7%，低表达组5年生存率为38.6%，两组5年生存率比较差异具有统计学意义（χ2=4.715，P=0.005）；多因素分析显示，临床分期、肿瘤进展、miRNA-21表达水平是影响哈萨克族食管癌患者预后的独立危险因素（P﹤0.05）。结论 miRNA-21在食管癌组织表达水平高于正常组织，且miRNA-21表达越高，患者预后越差，miRNA-21可以作为哈萨克族食管癌诊断及预后评价的指标之一。

Clinical significance of microRNA-21 expression level in esophageal cancer tissue in Kazakh

Objective To explore the clinical significance of miRNA-21 expression level in esophageal cancer in Ka-zakh. Method 72 samples of esophageal cancer tissues were collected from Kazakh patients, and the expression of miR-NA-21 in esophageal cancer tissues and adjacent normal tissues was detected by real-time quantitative PCR;Age, clinical stage, tumor location, differentiation, lymph node metastasis, and disease progression were summarized to analyze the correlation between miRNA-21 and those clinical measures. Median survival was utilized in evaluating the prognostic fac-tors of esophageal cancer in Kazakh. Result The relative expression of miRNA-21 in esophageal cancer tissue was (7.57±0.14), and was (1.37±0.08) in normal esophageal tissues, with significant difference observed (P<0.05). 72 cases of esophageal cancer patients were stratified as miRNA-21 high expression group (n=42) and low expression group (n=30), then it was found that miRNA-21 expression was related with clinical stage, lymph node metastasis, and tumor progres-sion, i.e., lymph node metastasis, higher miRNA-21 expression and more advanced clinical stage were associated with higher incidence of tumor progression (P<0.05). The 5-year survival rate was 20.7% in miRNA-21 high expression group, while was 38.6% in the low expression group, with statistically significant difference observed (χ2=4.715, P=0.005). Multivariate analysis revealed that clinical stage, tumor progression, miRNA-21 expression level were indepen-dent risk factors for Kazakh esophageal cancer patients (P<0.05). Conclusion Esophageal cancer tissues have higher miRNA-21 expression than normal tissues, and the expression level of miRNA-21 was negatively correlated with progno-sis, so miRNA-21 is a potential measure for the diagnosis and prognosis of Kazakh esophageal cancer patients.