人附睾蛋白4在非小细胞肺癌组织及血清中的表达及临床意义

目的 研究人附睾分泌蛋白4(HE4)在非小细胞肺癌(NSCLC)组织及血清中的表达及临床意义.方法 收集124例NSCLC患者(NSCLC组)的血液标本和肺癌组织标本,其中行手术治疗的患者取癌旁组织和正常肺组织,并在术后3天再次抽取血液标本;同期收集96例健康体检者(健康组)的血清标本.采用电化学发光免疫分析仪检测血清中HE4浓度;免疫组化EnVision二步法检测非小细胞肺癌组织、癌旁组织、正常肺组织中HE4的表达.结果 HE4在肺癌组织、肺腺癌组织、Ⅲ~Ⅳ期肺癌组织中的阳性表达率分别高于癌旁组织和正常肺组织、肺鳞癌组织、Ⅰ~Ⅱ期肺癌组织(P<0.05);NSCLC组患者的血清HE4浓度高于健康体检组(P<0.05);手术治疗的患者术后3天血清HE4浓度低于术前血清HE4浓度(P<0.05);NSCLC组患者血清HE4水平与病理分期、组织中HE4阳性表达呈正相关,与年龄、性别、病理类型无明显相关性.结论 NSCLC组织及血清HE4表达升高,可作为NSCLC的肿瘤标志物,是NSLCL的早期辅助诊断、疗效评价、预后评估的客观指标,可用于指导临床的综合治疗.

Expression and significance of human epididymis protein 4 in lung cancer tissue and serum of patients with non-small cell lung cancer

Objective To explore the expression and significance of human epididymis protein 4 (HE4) in lung can-cer tissue and serum of patients with non-small cell lung cancer (NSCLC). Method The blood and lung cancer tissue samples from 124 NSCLC patients (NSCLC group) were collected, tissues adjacent to cancer and normal lung tissues were additionally collected from patients administered with surgery, from whom the blood samples were collected again 3 days after surgery;At the same time, serum samples from 96 healthy subjects (control group) were collected. HE4 concen-tration in serum was detected by electrochemical luminescence immunoassay;HE4 expression in NSCLC tissues, tissues adjacent to lung cancer and normal lung tissues were detected by immunohistochemical assay of EnVision two step meth-od. Result The positive expression rates of HE4 in lung cancer tissues, lung adenocarcinoma tissues and lung cancer tis-sues at gradeⅢ-Ⅳ were significantly higher than that in tissues adjacent to lung cancer, normal lung tissues, lung squa-mous cell lung cancer tissues, and lung cancer tissues at gradeⅠ-Ⅱ(P<0.05);The serum level of HE4 was significantly higher in NSCLC patients than that in healthy subjects (P<0.05);The serum HE4 concentration in 3 days after surgery was significantly lower than that before surgery (all P<0.05);The level of serum HE4 in NSCLC patients was positively correlated with the pathological stage and the positive expression of HE4 in tissues, rather than with age, gender, or patho-logical type. Conclusion The elevated expression of HE4 in NSCLC tissues and serum can be a biomarker for NSCLC, and are potential objective indexes for early auxiliary diagnosis, evaluation of curative effect, and prognosis assessment of the carcinoma.