人支气管上皮细胞转化过程中凋亡与增殖的改变

目的 观察凋亡、增殖在人支气管上皮细胞转化中的改变，探讨其有关机理。方法 使用末端脱氧核苷酰转移酶标记凋亡法（ＴＤＴ）、Ｂｒｄｕ掺入法、Ｗｅｓｔｅｒｎ ｂｌｏｔｔｉｎｇ、荧光原位杂交等方法。结果 人永生化支气管上皮细胞Ｙ在无血清培养基连续转代过程中，晚代细胞软琼脂克隆形成率显著增高，并对血清分化产生明显抗性，对表皮生长因子（ＥＧＦ）依赖性有减弱倾向，细胞增殖速度明显加快，顺铂诱导的凋亡发生率显著低

Alterations of cell apoptosis and proliferation during transformation of a human bronchial epithelial cell line

Objective Toinvestigatethe alterations ofcell apoptosis and proliferation and its mechanism during transformation of a human bronchial epithelial cell line Y.Methods Terminal deoxyribonucleotidyl transferasein situlabelling,BrdUincorporation, Western blotting andfluorescenceinsitu hybridization were used inthe study.Results During serial passages ofthe immortalized human bronchial epithelial cellline Y,some phenotypic alterations, such as higher colony forming efficiency in soft agar, resistance to serum induced differentiation and low dependence on EGFwere observed. The increase in cell proliferation and decrease in cisplatinum induced apoptosisandthe enhanced protein expression of Bcl 2 , MDM2,PCNAand Cyclin D1 were foundinthelate passages ofthe cellline.The chromosomes of Ycells were diploid atthe early passages,but became aneuploidinthelate passages.Conclusion In additiontotheincreasein cell proliferation,inhibition of apoptosis mightalso contributetothe cytogenetic abnormality andthetransformation of human bronchial epithelial cells.