ELAM-1在鼻咽癌裸鼠移植瘤中的表达及其与转移的关系

目的 建立裸鼠鼻咽癌转移模型并探讨 E-选择素（ELAM-1）与鼻咽癌转移的相关性。方法 将鼻咽癌5-8F细胞悬液注射于裸鼠左后肢爪垫，观察裸鼠状态、成瘤情况并测量裸鼠体重及移植瘤长短径；采用连续病理切片苏木精-伊红染色观察移植瘤及转移情况，将16只人鼻咽癌荷瘤裸鼠分为转移组和非转移组；采用免疫组织化学法检测两组移植瘤组织中ELAM-1的表达。 结果 16只裸鼠均成瘤，成瘤率为100.0%，其中10只裸鼠出现转移瘤，转移率为62.5%。建模前，两组裸鼠体重差异无统计学意义［（13.83±0.56）g vs （14.62±0.30） g，t=1.026，P=0.071］。建模后4~7周，裸鼠瘤体体积呈指数增长，且转移组移植瘤增长速度较非转移组快，非转移组裸鼠瘤体体积小于转移组［（198.91 ± 163.29） mm³ vs （268.76 ±174.31） mm³，t=4.376，P=0.005］。ELAM-1在鼻咽癌裸鼠移植瘤、淋巴结转移灶及远处转移灶中的表达均为阳性，主要表达于细胞膜。转移组移植瘤光密度值高于非转移组（0.4497±0.0705 vs 0.0435±0.0082，t=4.388，P＝0.001）。结论 本研究成功构建稳定性好、转移率高的鼻咽癌裸鼠移植瘤转移模型，且ELAM-1在裸鼠移植瘤中高表达，可促进鼻咽癌裸鼠移植瘤生长和转移。

Expression of ELAM-1 in nasopharyngeal carcinoma xenografts in nude mice and its rela-tionship with metastasis

Objective To establish a model of metastatic nasopharyngeal carcinoma in nude mice and explore the correlation between ELAM-1 and metastasis of nasopharyngeal carcinoma. Methods Nasopharyngeal carcinoma 5-8F cell suspension was injected into the left hind paw pad of nude mice. Tumor formation，nude mice weight，the long and short diameter of the transplanted tumor were evaluated and tested. Sixteen human nasopharyngeal carcinoma-bearing nude mice were divided into metastasis group and non-metastasis group by continuous pathological section hematoxylin-eosin staining. ELAM-1 expression in transplanted tumor tissues was measured by immunohistochemistry. Results Tumor were formed in all mice，and the tumor formation rate was 100.0%. At last，ten nude mice showed metastatic tumors（metastatic rate，62.5%）. There was no significant difference in body weight between the two groups［（13.83±0.56） g vs （14.62±0.30） g，t=1.026，P=0.071） before the establishment of metastatic tumor model，then the tumor volume increased exponentially，and the growth rate of the tumors in the metastasis group was faster than that in the non-metastasis group. The tumor volume of the non-metastasis group was smaller than that of the metastasis group［（198.91 ± 163.29） mm³ vs （268.76 ±174.31）mm³ ，t=4.376，P=0.005）. ELAM-1 was positive in nasopharyngeal carcinoma xenografts，lymph node metastases and distant metastases，and mainly expressed in cell membrane. The optical density of transplanted tumors in the metastasis group was higher than that in the non-metastasis group（0.4497±0.0705 vs 0.0435±0.0082，t=4.388，P＝0.001）. Conclusions A metastatic model of nasopharyngeal carci-noma in nude mice with stability and high metastatic rate is successfully established. ELAM-1  is highly expressed in nasopharyngeal carcinoma xenografts in nude mice, promoting the growth and metastasis of transplanted tumors.