白细胞介素35和白细胞介素37与结直肠癌临床病理学特征及预后的相关性研究

背景与目的：结直肠癌（colorectal cancer，CRC）为世界第三常见恶性肿瘤，近年来研究者认为白细胞介素（interleukin，IL）-35和（或）IL-37与CRC的发展有关，但其作用机制尚未阐明。探究IL-35和IL-37在CRC发展中的作用及其可能的机制，分析IL-35和IL-37与CRC患者预后的相关性。方法：收集2013年—2017年在上海交通大学医学院附属同仁医院接受治疗的191例CRC患者手术病理蜡块的肿瘤组织，与其匹配的非癌组织是来源于同一患者的肠癌手术切缘蜡块组织。应用免疫组织化学（immunohistochemistry，IHC）染色法将CRC患者的癌组织与非癌组织染色，并运用Image-Pro Plus将IHC染色阳性部分定量分析，结合随访结果，探讨癌组织与非癌组织中IL-35和IL-37的表达水平与CRC临床病理学特征及预后的相关性。结果：与非癌组织相比，CRC组织中IL-35的表达量减少了50%（P<0.000 1）。CRC组织中IL-37的表达量与非癌组织相比增加了40%（P=0.012）。多因素生存分析显示，癌组织中IL-35（HR=0.39；95% CI：0.16~0.97；P=0.04）的表达水平是CRC患者术后生存的独立预测指标。结论：IL-35和IL-37蛋白的表达水平可能与CRC的发展有关，IL-35的表达水平可能是CRC患者术后生存的独立预测指标。

Correlation between interleukin 35 or interleukin 37 and clinicopathology and prognosis of colorectal cancer

Background and purpose: Colorectal cancer (CRC) is the third most common cancer in the world. Recently, it is suggested that interleukin (IL)-35 and/or IL-37 are associated with CRC, but the precise underlying mechanism has not been fully explored. The aim of this study was to explore the correlation between IL-35 or IL-37 and clinicopathology of CRC, and to determine whether IL-35 or IL-37 could be used as prognosis biomarker for CRC patients. Methods: Tissue array was generated from both CRC (n=191) and the matched non-cancer tissues (n=141) from the same patient’s adjacent tissues from 2013 to 2017 in Department of Pathology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine. Quantitative analysis for IL-35 and IL-37 was performed on these immunohistochemically (IHC) labelled tissues using Image-Pro Plus software, for comparison between cancer and non-cancer tissues. We investigated the correlation between IL-35/IL-37 in the CRC tissues and pathology, as well as prognosis. Results: The expression of IL-35 was decreased by 50% in CRC tissues compared with non-cancer tissues (P<0.000 1). In contrast, the expression of IL-37 was increased by 40% in CRC tissues compared with non-cancer tissues (P=0.012). Furthermore, the expression of IL-35, but not IL-37, in cancer tissues (HR=0.39, 95% CI: 0.16-0.97, P=0.04) was an independent predictor ofpostoperative survival in CRC patients, using the multivariate survival analysis. Conclusion: The expression of IL-35 or IL-37 may be involved in the development of CRC. The expression of IL-35 is believed to be an independent predictor of postoperative survival in CRC patients. The precise mechanism of IL-35 and IL-37 in the development of CRC in vivo will be determined in future.