CD151和MMP-7在结直肠癌中的表达及其临床意义

  目的  探讨人类白细胞分化抗原151（CD151）和基质金属蛋白酶-7（MMP-7）在结直肠癌中的蛋白表达，及其与结直肠癌发生、发展、转移的关系。  方法  采用免疫组织化学染色法检测结直肠癌组织中CD151和MMP-7的蛋白表达，阐明二者与患者临床病理特征之间的关系。  结果  CD151和MMP-7在大肠正常组织中表达的阳性率分别为5%（1/20）和15%（3/20），在结直肠癌组织中表达的阳性率分别为78%（39/50）和72%（36/50），CD151和MMP-7在大肠正常组织和结直肠癌组织中的表达差异有统计学意义（χ2=31.086，P<0.05；χ2=18.811，P<0.05）。两种蛋白的阳性表达率与结直肠癌患者的年龄、性别、部位无关联（P>0.05），但与淋巴结转移、浸润深度、远隔器官转移、肿瘤分化程度、Dukes分期有密切关系（P<0.05）。结直肠癌中CD151和MMP-7两种蛋白的表达强度具有相关性（r_s=0.314，P=0.026）。  结论  CD151和MMP-7在结直肠癌组织中异常高表达并与其发生、发展及浸润转移有密切关系，联合检测可作为判断结直肠癌生物学行为的重要指标。 

CD151 and MMP-7 Expression and Its Significance in Colorectal Cancer

  Objective  To evaluate the expression levels of CD151 and matrix metalloproteinase-7 (MMP-7) in colorectal cancer and their relationships with the occurrence, development, metastasis, and prognosis of colon cancer.  Methods  The expression levels of CD 151 and MMP-7 proteins in cancer tissue microarray were determined by the immunohistochemical SP method. Their correlations with clinicopathologic features were analyzed.  Results  The positive rates of CD151 and MMP-7 expression was 0.5% (1/20) and 15% (3/20) in normal tissue, as well as 78% (39/50) and 72% (36/50) in colon cancer tissues, respectively. The expression rates of the two types of protein were significantly different between the two groups (χ2 = 31.086, P < 0.05; and χ2 = 18.811, P < 0.05). The expression levels of the two proteins in colorectal cancer tissues were not associated with patient age, gender, or tumor location (P > 0.050), but were significantly related with the differentiation degree of cancer tissue, depth of invasion, organ metastasis, lymph node metastasis, and Duke's staging (P < 0.050). There was a positive correlation between CD151 and MMP-7 expression (r_s = 0.314, P = 0.026).  Conclusion  CD 151 and MMP-7 are overexpressed in colon carcinoma, suggesting that these two proteins are related to carcinogenesis, development, invasion, and metastasis. The joint detection of these two makers can be used to determine the biological behavior and prognosis of colon cancer.