碘化钾制备的碘涂层钛板的抗菌性能研究

目的探讨利用碘化钾制备碘涂层钛板的可行性,并验证其抗菌性能。方法以碘化钾作为电解液,使用电泳沉积法将碘负载到钛板表面,制备出碘涂层钛板。通过扫描电镜与能谱分析,观察碘涂层钛板的表面征象和成分结构。实验分组:对照组是经过预处理但未行载碘的钛板,共10块;实验组是经过预处理后再行载碘的碘涂层钛板,根据电解液浓度的不同分为3组:1000 mg/L、2000 mg/L、4000 mg/L组,每组各10块。使用1×106 CFU/mL的金黄色葡萄球菌(ATCC25923)进行体外抗菌实验,并对其抗菌性能进行研究。结果碘涂层钛板外观呈灰色,表面可均匀覆盖一层平整、无塌陷的涂层,电镜下见其表面可形成碘涂层,局部散在分布大小不等的不规则陷凹。对照组、1000 mg/L组、2000 mg/L组、4000 mg/L组的钛板碘含量分别为0、5.10、10.32、15.05 mass%;其体外抗菌菌落计数分别为56.00±5.09、21.40±2.76、9.10±2.51和2.00±1.88,4组之间菌落计数比较差异均有统计学意义(P<0.05)。结论碘化钾作为电解液,使用电泳沉积法可成功制备出碘含量稳定、涂层分布均匀的碘涂层钛板。体外抗菌实验证明碘涂层钛板的抗菌性能强于未载碘钛板。

An iodine-coated titanium plate prepared with potassium iodide

Objective To explore the feasibility of preparing an iodine-coated titanium plate with potassium iodide and verify its antibacterial performance.Methods Iodine was coated onto the surface of a titanium plate in electrolyte of potassium iodide using the electrophoretic deposition method.The signs and composition of the surface of the iodine-coated titanium plate were observed by scanning electron microscopy and energy dispersive spectroscopy.The experiment was conducted in a control group and 3 antibacterial test groups.The control group consisted of 10 titanium plates which had been pretreated but not loaded with iodine;the 3 experimental groups also had in each 10 titanium plates which had been pretreated and loaded with iodine in the electrolytes of concentrations of 1,000 mg/L,2,000 mg/L and 4,000 mg/L,respectively.The antimicrobial tests in vitro were conducted with standard strains of staphylococcus aureus1×106 Colony-Forming Units(CFU)/mL ATCC25923]to determine the antibacterial property of the plates.Results The iodine-coated titanium plates appeared grey and their surface was evenly covered with a flat coating with no collapse.The scanning electron microscopy observed on the surface of the iodine-coated titanium plates an iodine coating with scattered irregular collapses in different sizes.The iodine content was 0 mass%,5.10 mass%,10.32 mass%and 15.05 mass%,respectively,in the control,1,000 mg/L,2,000 mg/L and 4000 mg/L groups under the energy dispersive spectroscopy.Their counts of in vitro antibacterial colony were 56.00±5.09,21.40±2.76,9.10±2.51,and 2.00±1.88,respectively,showing significant differences between groups(P<0.05).Conclusions A titanium plate with a steady and even iodine coating can be prepared by virtue of the electrophoretic deposition method in electrolyte of potassium iodide.The antibacterial property of an iodine-coated titanium plate is superior to that of a titanium plate without iodine coating.