静电纺丝壳聚糖-明胶复合纤维的制备和体外生物相容性评价 |
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引用本文: | 何晨光,赵莉.静电纺丝壳聚糖-明胶复合纤维的制备和体外生物相容性评价[J].组织工程与重建外科,2012,8(6):316-322. |
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作者姓名: | 何晨光 赵莉 |
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作者单位: | 上海组织工程研究与开发中心;组织工程国家工程研究中心 |
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基金项目: | 上海市自然科学基金项目(10ZR1424400) |
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摘 要: | 目的采用静电纺丝技术制备壳聚糖-明胶复合纤维,体外评价其生物相容性。方法采用扫描电镜(SEM)观察纤维支架的表面形貌,考察纺丝溶液浓度、黏度以及壳聚糖/明胶质量比对纤维直径和结构的影响。将人成纤维细胞接种在纤维支架表面,绘制细胞生长曲线,观察细胞在支架表面的形态。结果通过静电纺丝技术,制备的壳聚糖-明胶复合纤维均匀、光滑,无珠状结构,纤维平均直径为600 nm~1.6μm。随着纺丝溶液浓度和黏度增加,纤维平均直径增大;随着壳聚糖/明胶质量比增加,纤维直径也随之增大。成纤维细胞在复合纤维支架上生长良好,增殖快,并且保持较好的成纤维细胞形态。结论通过静电纺丝技术制备的壳聚糖-明胶复合纤维具有良好的生物相容性,有望作为皮肤组织工程的支架材料。
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关 键 词: | 静电纺丝 壳聚糖-明胶复合纤维支架 纤维直径 人成纤维细胞 |
Fabrication and Biocompatibility of Electrospun Chitosan-Gelatin Composite Fibers in Vitro |
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Authors: | HE Chenguang ZHAO Li |
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Affiliation: | Shanghai Tissue Engineering Research and Development Center,National Tissue Engineering Research Center,Shanghai 200241,China |
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Abstract: | Objective To fabricate the electrospun chitosan/gelatin composite fibers by the electrospinning technique and to investigate the biocompatibility of chitosan/gelatin composite fibers in vitro. Methods The surface morphology of the fibrous scaffolds was observed using scanning electron microscope (SEM). The effect of solution concentration, viscosity, chitosan/gelatin mass ratio on the fiber diameter and microstructure were investigated. The human fibroblasts were seeded on the fibrous scaffolds and cultured for 7 days. After the culture of different periods, the growth curve was obtained and the cell-scaffold composite was observed by SEM. Results In this study, the chitosan-gelatin composite fibers were fabricated by the electrospinning technique with homogeneous, smooth fibers without beads. The average fiber diameter was in the range of 600 nm-1.6 μm. The average fiber diameter increased with the increase of concentration and viscosity, meanwhile, with the increase of chitosan/gelatin mass ratio. The human fibroblasts grew fast and maintained spindle-shape morphology. Conclusion The chitosan-gelatin composite fibers fabricated using the electrospinning technique have good biocompatibility and are potentially used as skin tissue engineered scaffold materials. |
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Keywords: | Electrospinning Chitosan-gelatin composite fibrous scaffold Fiber diameter Human fibroblast |
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