| 转血管内皮细胞生长因子基因干细胞结合珊瑚羟基磷灰石修复兔骨缺损的实验研究 |
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| 引用本文： | 卢向东,刘强,贾祎佳,韩树峰,李平,高宏,吴斗,孙海飚,李钢.转血管内皮细胞生长因子基因干细胞结合珊瑚羟基磷灰石修复兔骨缺损的实验研究[J].中华关节外科杂志（电子版）,2011,5(1):53-59. |
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| 作者姓名： | 卢向东 刘强 贾祎佳 韩树峰 李平 高宏 吴斗 孙海飚 李钢 |
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| 作者单位： | 山西医科大学第一临床医学院骨科,太原,030001 |
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| 摘 要： | 目的探讨珊瑚羟基磷灰石（CHA）结合转血管内皮细胞生长因子（VEGF）基因的骨髓间充质干细胞构建的组织工程骨修复兔骨缺损的效果。方法 以CHA作为转VEGF基因骨髓间充质干细胞（BMSCs）的可吸附载体,体外构建组织工程骨,将其植入兔尺骨中段10mm骨缺损处,以CHA/BMSCs、自体骨移植组和空白组作为对照,在术后2、4、8、12周进行大体解剖、X线摄片、病理组织切片、生物力学测试,观察各组骨愈合程度及力学强度。结果 术后8周病理组织切片及X线摄片显示实验组骨缺损基本修复,效果优于对照组。生物力学测试显示术后12周组织工程骨组组抗扭转强度优于自体骨移植组。结论 CHA结合转VEGF基因BMSCs构建组织工程骨可用于骨缺损修复,优于自体骨移植组。
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| 关 键 词： | 受体，血管内皮生长因子 基因转移技术 组织工程 |
Application of CHA with VEGF transfected BMSCs in the repair of segmental ulnar defects in rabbits |
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| LU Xiang-dong,LIU Qiang,JIA Yi-jia,HAN Shu-feng,LI Ping,GAO Hong,WU Dou,SUN Hai-biao,LI Gang.Application of CHA with VEGF transfected BMSCs in the repair of segmental ulnar defects in rabbits[J].Chinese Journal of Joint Surgery(Electronic Version),2011,5(1):53-59. |
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| Authors: | LU Xiang-dong LIU Qiang JIA Yi-jia HAN Shu-feng LI Ping GAO Hong WU Dou SUN Hai-biao LI Gang |
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| Affiliation: | LU Xiang-dong,LIU Qiang,JIA Yi-jia,HAN Shu-feng,LI Ping,GAO Hong,WU Dou,SUN Hai-biao,LI Gang.Department of Orthopaedics,First Clinical Medical College,Shanxi Medical University,Taiyuan 030001,China |
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| Abstract: | Objective To investigate the efficacy of combined use of CHA and VEGF transfected BMSCs for repairing segmental bony defects.Methods CHA/VEGF/BMSCs composites were prepared and implanted in continuous defects in ulna of adult rabbits,while CHA/BMSCs,autograft implantation and no graft were used as controls.The animals were sacrificed on 2,4,8 and 12 weeks after operation.The bone repair process was investigated using physical,roentgenographical,vascularization,histological and biomechanical methods.Results ... |
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| Keywords: | Receptors vascular endothelial growth factor Gene transfer techniques Tissue engineering |
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