| CD74基因调控CXCR4抑制肾癌786-O细胞的侵袭作用 |
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| 引用本文： | 纪世琪,赵玉千,韩志兴,张海建,程文龙.CD74基因调控CXCR4抑制肾癌786-O细胞的侵袭作用[J].现代泌尿外科杂志,2014(2):111-113. |
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| 作者姓名： | 纪世琪 赵玉千 韩志兴 张海建 程文龙 |
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| 作者单位： | 首都医科大学附属北京地坛医院,北京100015 |
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| 基金项目： | 2013年首都医科大学基础临床科研合作课题（No.1300171706） |
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| 摘 要： | 目的探讨抑制人CD74基因后影响肾癌786-O细胞侵袭能力的作用机制。方法通过慢病毒lentivirus-CD74转染肾癌786-O细胞后,MTT法观察细胞活力变化,Transwell实验观察细胞侵袭能力的变化,应用Westernblot技术检测CD74、CXCR4蛋白的变化情况。结果抑制CD74蛋白表达水平后肾癌786-O细胞活力降低,侵袭能力降低,同时伴有CXCR4蛋白的表达降低。结论通过慢病毒转染抑制CD74的表达可以抑制肾癌细胞的侵袭能力,CD74基因可能是通过调控CXCR4蛋白的变化影响细胞侵袭能力的。
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| 关 键 词： | 肾癌 CD74 CXCR4 侵袭 |
CD74 inhibits 786-O cell invasion in renal cancer by modulating CXCR4 expression |
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| JI Shi-qi,ZHAO Yu-qian,HAN Zhi xing,ZHANG Hai-jian,CHENG Wen-long.CD74 inhibits 786-O cell invasion in renal cancer by modulating CXCR4 expression[J].Journal of MOdern Urology,2014(2):111-113. |
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| Authors: | JI Shi-qi ZHAO Yu-qian HAN Zhi xing ZHANG Hai-jian CHENG Wen-long |
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| Affiliation: | (Department of Urology, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China) |
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| Abstract: | Objective To study Methods Lentivirus-CD74 was used to the effect and mechanism of CD74 in inhibiting 786-0 cell invasion in renal cancer transfect renal cancer 786-0 cells,cell vitality was observed with MTT assay,cell inva- sion was observed with transwell assay, and the expressions of CD74 and CXCR4 were detected with Westernblot. Results The transfected lentivirus-CD74 786-0 ceils induced decreased ceil proliferation and invasion,and at the same time, the expres- sion of CXCR4 protein reduced. Oonclusions Transfecting CD74 with lentivirus can inhibit the cell invasion of renal cancer, indicating that CD74 may affect the cell invasion by regulating CXCR4 expression. |
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| Keywords: | renal cancer CD74 CXCR4 invasion |
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