两种相变型多功能纳米粒的制备及体外特性比较

目的制备包裹吲哚菁绿(ICG)及不同相变材料全氟正戊烷(PFP)或全氟己烷(PFH)]的两种相变型多功能纳米粒,比较其理化性质、稳定性及体外相变特性。方法采用改良的双乳化法制备包裹ICG及液态PFP或PFH的乳酸-羟基乙酸(PLGA)纳米粒(分别称为IPNPs、IHNPs),比较两者的一般特性、稳定性及热致相变、光致相变、声致相变和光声成像能力,采用MTT法检测光致相变所需能量的细胞毒性。结果IPNPs及IHNPs粒径分别为(490.53±36.96)nm及(512.23±38.52)nm,电位分别为(-14.40±1.40)mV及(-13.97±1.61)mV,二者差异无统计学意义(P均0.05);二者形态、吸收光谱也均无明显差异,于37℃储存24h均较稳定;IPNPs热致相变、光致相变及声致相变所需能量均低于IHNPs,且IPNPs光声及超声成像增强能力高于IHNPs。经能使IPNPs及IHNPs发生相变的激光能量辐照后,MH7A细胞存活率分别为(95.34±7.96)%,(54.92±6.11)%,二者差异有统计学意义(P0.05)。结论 IPNPs是更适于诊疗一体化应用的多功能纳米粒。

Preparation and characteristic comparison of two phase-transition multifunctional nanoparticles

Objective To prepare two multifunctional nanoparticles loaded with indocyanine green (ICG) and liquid fluorocarbon (perfluoropentane PFP] or perfluorohexane PFH]), and compare their physicochemical properties, stability phase-transition and imaging capacity in vitro. Methods The PLGA nanoparticles loaded with ICG and PFP or PFH (IPNPs or IHNPs) were prepared using a modified double emulsion technique, and then the physicochemical properties, stability and phase-transition under heating, laser irradiation, LIFU exposure and photoacoustic imaging capacity were compared. Results There were no significant differences between the IPNPs and IHNPs about their physicochemical properties, including size (490.53±36.96] nm vs 512.23±38.52] nm), zeta potential (-14.40±1.40] mV vs -13.97±1.61] mV) and absorption spectrum, and both of them were stable within 24 h. The phase-transition energy under different situations was significantly lower in IPNPs group compared with IHNPs. In addition, the ultrasonic and photoacoustic imaging capacity obviously increased in IPNPs. The cell viability was higher in IPNPs phase-transition irradiation with laser energy than that in IHNPs (95.34±7.96]% vs 54.92±6.11]%, P<0.05). Conclusion IPNPs might be a more promising multifunctional platform for theranostics.