异丙酚对大鼠脑缺血再灌注后脑细胞凋亡和Bcl-2、Caspase-3蛋白表达的影响

脑缺血再灌注后脑损伤的发病机制较复杂,其所致病理损伤包括细胞坏死和凋亡。细胞凋亡与脑缺血再灌注损伤关系密切,是神经元迟发性死亡的主要形式。研究提示与凋亡密切相关的Bcl-2、Caspase-3蛋白等参与了这种迟发性死亡,它们表达的量决定了细胞的生存。本实验采用流式细胞仪、

The effect of propofol on cell apoptosis and expression of Bcl-2 and caspase-3 protein of focal cerebral ischemia -reperfusion in the rats

Objective To investgate the effects of propofol on cell apoptosis of focal cerebral ischemia-reperfusion injury in the rat model of reversible middle cerebral artery occlusion(MCAO).Methods Male SD rats were used to undergo 2 h MCAO and 2 h reperfusion 2.Brain cell apopotosis and expression of bcl-2 protein was measured by flow cytometry(FCM),expression of caspase-3 protein was observed by immunohistochemical method;brain cell structure was examined under light microscope.Results Propofol could significantly inhibit the apoptosis of brain cell after focal cerebral ischemia-reperfusion.Compared to sham group,caspase-3 protein positive-staining cells were remarkably increased in control group,while propofol group were remarkably decreased.Pretreatment of animals with propofol before reperfusion significantly increased the Fluorescent index of Blc-2.Conclusion The increased expression of Caspase-3 may be an important mechanism for brain cell apoptosis after brain ischemia-reperfusion.Propofol possesses the antiapoptotic effects which may be related to the reduction of the expreesion of caspase-3 and increasing the expression of Bcl-2 protein.