| 人精子与溶脲脲原体的共同抗原 |
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| 引用本文： | 叶元康,陆德源,朱云凤,彭伟,王一飞.人精子与溶脲脲原体的共同抗原[J].中国男科学杂志,1994(1). |
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| 作者姓名： | 叶元康 陆德源 朱云凤 彭伟 王一飞 |
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| 作者单位： | 上海第二医科大学微生物学教研室
(叶元康，陆德源)，上海第二医科大学免疫学教研室
(朱云凤，彭伟)，上海第二医科大学免疫学教研室(王一飞) |
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| 摘 要： | 溶脲脲原体(UU)是人泌尿生殖道的常见病原体,男性感染者又常伴有抗精子抗体,并可致不育。本实验证实了人精子与UU有共同抗原。① ELISA方法抗 UU抗体不仅可与UU抗原起反应,也可与人精子或精子膜发生反应;抗人精子抗体同样也可与UU抗原发生反应;②10％不连续SDS-PAGE可见人精子、精子膜提取物与UU有相同的蛋白电泳区带;③免疫印迹技术证明抗UUIgG对转移到硝基纤维薄膜上的人精子、精子膜和UU的电泳条带,在分子量 61KDa处发生特异性的交叉反应。
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| 关 键 词： | 溶脲脲原体 人精子 共同抗原 |
STUDY ON THE COMMON ANTIGEN BETWEEN HUMAN SPERM AND UREAPLASMA UREALYTICUM |
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| YE Yuan-Kang,et al.STUDY ON THE COMMON ANTIGEN BETWEEN HUMAN SPERM AND UREAPLASMA UREALYTICUM[J].Chinese Journal of Andrology,1994(1). |
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| Authors: | YE Yuan-Kang |
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| Affiliation: | YE Yuan-Kang,et al
Department of Microbiology,Shanghai second Medical University |
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| Abstract: | Ureaplasma urealyticum(UU)is a common pathogen in the human urogenital tract. The UU infected males often produced anti-sperm antibody and caused infertility. Our experiments demonstrated that there were presence of common antigen between human sperm and UU. The evidences were : (1) cross-immune reaction was present between anti-UU serum and human sperm or sperm membrane protein and vice versa$(2) Among UU, human sperm and sperm membrane protein,there were several similar protein electrophoresis bands in 10% discon-tinuons SDS-PAGE; (3) Among UU, human sperm and sperm membrane protein, there was similar 61 KD protein on nitrocellulose membrane in Western blotting by using of anti-UU IgG. |
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| Keywords: | Ureaplasma urealyticum Human sperm Common antigen |
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