| 小鼠iPS细胞具备诱导性原始生殖细胞分化潜能的研究 |
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| 作者姓名： | Guo X Zhang YM Qi ZY Qin J Cui GH Gui YT Cai ZM |
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| 作者单位： | 1. 北京大学深圳医院男性生殖和遗传广东省重点实验室,广东深圳,518036
2. 深圳北京大学香港科技大学医学中心,广东深圳,518036 |
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| 基金项目： | 深圳市科技研发资金基础研究计划(JC201005260214A); 广东省医学科研基金(B2009230); 深圳市科技计划重点项目(201001014); 高等学校博士学科点专项科研基金(20090001120130)~~ |
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| 摘 要： | 目的:探讨小鼠诱导性多能干细胞IP14D-1是否具备诱导性原始生殖细胞(induced primordial germcells,iPGCs)分化潜能,以及特异基因表达变化及可能机制。方法:未分化IP14D-1培养扩增,分化形成诱导性拟胚体(induced embryoid bodies,iEBs)。RT-PCR和免疫荧光分别检测4、7、9 d的iEBs中Lin28、Blimp1、Stra8和Mvh的表达变化和蛋白定位情况。结果:未分化IP14D-1同小鼠胚胎干细胞(mouse embryonic stem cells,mESCs)相同,Lin28表达较弱,Blimp1表达相对较强,Mvh和Stra8也在这两种细胞及其相应iEBs和EBs中表达,但均无明显差别。IP14D-1分化形成的iEBs从4 d生长到7 d时,Lin28表达逐渐增强,到9 d时表现为下降,Blimp1表达则随着iEB生长时间延长而逐渐降低。结论:建立了IP14D-1和相应拟胚体(iEBs)的完善、稳定的培养及分化体系;未分化IP14D-1与mESCs在Lin28、Blimp1、Mvh和Stra8表达方面无明显差别;iEB和EBs的Mvh和Stra8表达也无明显差别。IP14D-1及iEBs具有iPGCs分化潜能,且可能是7 d的iEBs内iPGCs分化数量较多,之后进入iPGCs分化的Lin28低表达时期。
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| 关 键 词： | 诱导性多能干细胞 原始生殖细胞 Lin28 Blimp1 小鼠 |
Mouse-induced pluripotent stem cells have the potential to differentiate into induced primordial germ cells |
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| Guo X,Zhang YM,Qi ZY,Qin J,Cui GH,Gui YT,Cai ZM.Mouse-induced pluripotent stem cells have the potential to differentiate into induced primordial germ cells[J].National Journal of Andrology,2011,17(11):966-972. |
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| Authors: | Guo Xin Zhang Yan-min Qi Zheng-yu Qin Jie Cui Guang-hui Gui Yao-ting Cai Zhi-ming |
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| Affiliation: | GUO Xin1,ZHANG Yan-min1,QI Zheng-yu1,QIN Jie1,CUI Guang-hui1,GUI Yao-ting1,CAI Zhi-ming2 1.Key Laboratory of Male Reproduction and Genetics of Guangdong Province,Shenzhen Hospital of Peking University,Shenzhen,Guangdong 518036,China,2.Shenzhen PKU-HKUST Medical Center |
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| Abstract: | Objective: To investigate whether mouse-induced pluripotent stem(iPS) cell line IP14D-1 has the potential to differentiate into induced primordial germ cells(iPGCs),and to explore the changes in the expression of iPGCs-differentiation associated genes and their possible mechanisms.Methods: Undifferentiated IP14D-1 was cultured to proliferate and then differentiated to form 4-,7-and 9-day-old induced embryoid bodies(iEBs) in vitro,respectively.RT-PCR and immunofluorescence were used to detect the expressions... |
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| Keywords: | mouse-induced pluripotent stem cell primordial stem cell Lin28 Blimp1 |
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