口腔鳞状细胞癌中心体扩增与染色体不稳定的相关性研究

目的 探讨口腔鳞状细胞癌(oral squamous cell carcinomas,OSCC)染色体不稳定形成的潜在机制.方法 采用间接免疫荧光染色法观察8例正常口腔黏膜及32例OSCC石蜡包埋组织的中心体形态及数目,采用流式细胞术检测相应组织DNA倍性情况,分析中心体异常与非整倍体之间的相关性.结果 8例正常口腔黏膜均显示大小数目正常的中心体,32例OSCC组织中,25例有明显的中心体数目扩增及排列紊乱,21例为非整倍体;中心体扩增发生率在非整倍体OSCC组(19/21)高于二倍体OSCC组(6/11)(Fisher确切概率=0.032);相关分析显示中心体扩增与OSCC非整倍体之间存在相关关系(Spearman r=0.413,P=0.019),中心体扩增程度与核型异常程度之间呈直线正相关关系(Pearson r=0.364,P=0.041).结论 中心体扩增作为有丝分裂过程中染色体分离错误的始动因素,可能是OSCC非整倍体形成的潜在原因之一.

Centrosome amplification and chromosome instability in oral squamous cell carcinomas

OBJECTIVE: To investigate the relationship between centrosome abnormalities and aneuploidy in oral squamous cell carcinoma (OSCC) and elucidate the possible underlying mechanisms of chromosome instability (CIN) in OSCC. METHODS: Formalin-fixed, paraffin-embedded tissues of 8 cases of normal oral epithelium and 32 cases of OSCC were examined for centrosome status by using indirect immunofluorescence staining, and chromosome instability (aneuploidy) in some tissues were detected by flow cytometry. The correlation between centrosome abnormalities and aneuploidy in OSCC was statistically analyzed by SPSS12.0. RESULTS: Normal oral epithelium showed normal size and number of centrosomes in epithelium cells, while 25 out of 32 cases of OSCC showed the evident centrosome amplification characterized by huge size and/or supernumerary centrosomes in a fraction of tumor cells, and 21 out of 32 cases were aneuploidy. The percentage of cases with abnormal centrosomes in aneuploid OSCC (19/21) was significantly higher than that in diploid OSCC(6/11) (P =0.032). Centrosome abnormality was significantly correlated with aneuploidy (Spearman r = 0.413, P = 0.047), and a positive correlation was found between the degree of centrosome amplification and the degree of DNA ploidy abnormality (Pearson r = 0.364, P = 0.041). CONCLUSIONS: Centrosome abnormality may be a contributing factor for chromosome instability in OSCC.