伴放线放线杆菌在龈下菌斑和颊黏膜分布研究

目的:比较伴放线放线杆菌(actinobac illus actinomycetem com itans,A.a)在不同类型牙周炎患者龈下菌斑和颊黏膜中的分布。方法:通过聚合酶链反应(polym erase chain reaction,PCR)对侵袭性牙周炎患者(AgP)、慢性牙周炎患者(CP)、牙周健康者口腔龈下菌斑和颊黏膜中的A.a进行检测,分析该菌分别在两部位的相对含量。结果:AgP组菌斑和颊黏膜样本中A.a阳性检出率均为41.7%,分别高于CP组(菌斑16.7%、颊黏膜10.0%)和牙周健康组(菌斑和颊黏膜均为0%)。AgP组A.a在菌斑和颊黏膜的相对含量分别为38.5%和22.2%,高于CP组(菌斑19%、颊黏膜12.75%)。结论:A.a不仅存在于龈下菌斑中,也能够粘附于颊黏膜;A.a是AgP的主要优势菌也参与了CP的菌群组成。

Occurrence of Actinobacillus Actinomycetemcomitans in Buccal Mucosa and Subgingival Plaque from the Chronic and Aggressive Periodontitis Patients

Objective: To determine the distribution patterns of Actinobacillus actinomycetemcomitans(A.a) in buccal mucosa and subgingival plaque among different periodontitis.Methods: A.a in buccal mucosa and subgingival plaque from patients with aggressive periodontitis(AgP),chronic periodontitis(CP) and periodontally healthy subjects was detected by PCR.The relative quantity of A.a in two sites was analyzed.Results: A.a was detected in a higher percentage of AgP groups(41.7% respectively) in both subgingival microbial samples and buccal mucosa samples than that of CP groups(subgingival plaque 16.7%,buccal mucosa 10.0%) and periodontally healthy subjects(0% respectively).The relative quantity of A.a was 38.5% in subgingival microbial samples and 22.2% in buccal mucosa samples in the AgP groups,which were higher than CP groups(subgingival plaque 19%,buccal mucosa 12.75%).Conclusion: A.a can implant not only in subgingival plaque but also in buccal mucosa.A.a is one of the important putative periodontopathogens in AgP groups and part of subgingival flora in CP.