NS-398对Tca8113细胞体外增殖的抑制作用

目的:探讨环氧合酶-2抑制剂NS-398对Tca8113细胞的生长抑制作用。方法:应用四甲基偶氮唑蓝(MTT)快速比色法,观察NS-398对Tca8113细胞生长的抑制作用,检测NS-398与Tca8113细胞间的时-效关系和量-效关系;通过流式细胞仪(FCM)研究NS-398对Tca8113细胞周期的影响和作用,采用SAS8.1统计软件包进行数据处理,均数比较采用t检验和重复测量方差分析。结果:含0、25、50、75、100、125、150、200μmol/LNS-398的Tca8113细胞培养液,在分别培养48、72、96、120h后,随着作用时间的延长和药物浓度的增加,抑制作用也增强。NS-398在浓度150μmol/L作用96h后,抑制作用明显(药物浓度:P<0.05;时间:P<0.001),NS-398可引起Tca8113细胞G0/G1期细胞的大量增加,S期和G2/M期细胞比例数减少,阻滞细胞生长于G0/G1期(P<0.001)。结论:NS-398可抑制Tca8113细胞的增殖,并具有时间和浓度依赖性;这种作用可能与阻止细胞周期进展有关,NS-398可能在口腔鳞癌治疗中发挥重要作用。

The inhibitive effect of COX-2 inhibitor NS-398 on cancer cell growth of human tongue squamous cell carcinoma

PURPOSE: To investigate the inhibitive effect of COX-2 inhibitor NS-398 on cancer cell growth of human tongue squamous cell carcinoma. METHODS: MTT assay was used to study the inhibitive effect of NS-398 on the growth of Tca8113 cells, and to investigate the time-effect relationship and dose-effect relationship between NS-398 and Tca8113 cells. The effect of NS-398 on cell cycle changes of Tca8113 was studied by flow cytometry (FCM). The statistical date were analysed by SAS 8.1 software. Student's t test and repeated-measure ANOVA were used to analyze the date. RESULTS: The Tca8113 cell culture median contained 0, 25, 50, 75, 100, 125, 150, 200 micromol/L NS-398, and individually cultured for 48, 72, 96, 120 hours. The inhibitive effect increased with the time prolonging and the concentration increasing. The effect was most obvious with 150 micromol/L NS-398 cultured for 96 hours (concentration: P<0.05, time: P<0.001). NS-398 can cause great increase of Tca8113 cells in G0/G1 phase, while cells in G2/M phase decreased, and cells arrested in G0/G1 phase (P<0.001). CONCLUSION: NS-398 can inhibit the growth of Tca8113 cells. The inhibitive effect of NS-398 on Tca8113 cells is dose-dependent and time-dependent, and this effect may be related to the inhibition of cell circle growth. NS-398 may play an important role in the treatment of oral squamous cell carcinoma.