细胞凋亡抑制基因bcl-2和survivin在血管瘤不同时期的表达

目的:探讨血管瘤自然消退过程与细胞凋亡的关系.方法:收集血管瘤及血管畸形标本63例,均经病理证实;所有患者均未经过激素或其他药物治疗.其中血管瘤44例,按照组织学特征将其分为增殖期23例,退化期21例;血管畸形19例.每例标本采用SABC法免疫组化,检测bcl-2、survivin 2种细胞凋亡抑制基因的蛋白表达,同时以8例正常皮肤组织作为对照.采用SPSS11.0软件包行×列表卡方检验.结果:血管瘤增殖期和退化期的bcl-2表达不同,具有显著性差异(P＜0.01);而survivin的表达无显著性差异(P＞0.05).结论:Bcl-2在血管瘤增殖期血管内皮细胞的高表达与退化期表达有显著性差异.提示该基因可能在血管瘤增殖期抑制内皮细胞凋亡方面发挥作用;而survivin在血管瘤增殖期内皮细胞的高表达,与退化期表达无显著性差异,提示该基因与血管瘤的自然消退关系不大.

Expression of bcl-2 and survivin gene products in different stages of vessel differentiation in hemangiomas

PURPOSE: To investigate the relationship between cell apoptosis and regression process of hemangiomas. METHODS: Fourty four hemangiomas and 19 vascular malformations, confirmed by pathology without any previous treatments were involved. Hemangiomas were classified into two groups by histological characters, among which 23 were in proliferating phase and 21 cases were in involuting phase. SABC immunohistochemistry was used to determine the expression of bcl-2 and survivin genes in vascular and endothelial cells,and 8 normal skin tissues were obtained from infants and children for control. The data was analyzed using Chi-square test with SPSS 11.0 software package. RESULTS: Significant difference of bcl-2 expression in endothelial cells was detected between the proliferation and involuting phase of hemangiomas (P<0.01). There was no significant difference of survivin expression in endothelial cells between the proliferating and involuting phases of hemangiomas. CONCLUSION: This study confirms high level of bcl-2 expression in vascular endothelial cells in proliferating phase of infantile hemangiomas in contrast to that in the involuting phase of hemangiomas, which indicates that bcl-2 might play a role in the later stage of angiogenesis. While high level of survivin expression in vascular endothelial cells in the proliferating phase and involuting phase without significant difference indicates that survivin might not play a role in the apoptosis of infantile hemangiomas.