| atRA对C57BL/6N小鼠腭突间充质细胞周期分布的影响及其作用机制 |
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| 引用本文： | 汪淼,黄洪章,侯劲松.atRA对C57BL/6N小鼠腭突间充质细胞周期分布的影响及其作用机制[J].中国口腔颌面外科杂志,2010,8(2):171-177. |
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| 作者姓名： | 汪淼 黄洪章 侯劲松 |
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| 作者单位： | 中山大学附属口腔医院,口腔颌面外科,广东,广州,510056 |
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| 摘 要： | 目的：在全反式维A酸（all-trans retinoic acid,atRA）诱导建立腭裂小鼠模型的基础上,检测胎鼠体内胚胎腭突间充质细胞的周期分布,并检测相关周期蛋白的变化情况,为进一步阐明维A酸（retinoic acid,RA）诱导腭裂机制提供新的线索。方法：以atRA建立C57BL/6N胎鼠腭裂模型,采用流式细胞术及免疫组化检测小鼠胚胎腭突间充质细胞的周期分布,通过实时定量RT-PCR和Western印记法检测p21和pRb在胎鼠胚胎腭突间充质中的mRNA和蛋白表达水平。采用SPSS11.0软件包对数据进行单因素方差分析和t检验。结果：流式细胞术及免疫组化检测发现,在小鼠妊娠日（gestation day,GD）第10天时给予atRA,可以诱导胎鼠胚胎腭突间充质细胞在一定妊娠阶段出现G1期细胞周期阻滞,同时也使胎鼠胚胎腭突间充质内p21和pRb的表达水平出现改变。而妊娠第12天（GD12）时给予atRA则无此现象。结论：p21与pRb参与了GD10时给予atRA诱导组胎鼠胚胎腭突间充质细胞G1期阻滞的发生。
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| 关 键 词： | 全反式维A酸 胚胎腭间充质细胞 p21 pRb 细胞周期 |
The mechanism of cell-cycle arrest induced by atRA in mouse embryonic palate mesenchymal cells |
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| WANG Miao,HUANG Hong-zhang,HOU Jin-song.The mechanism of cell-cycle arrest induced by atRA in mouse embryonic palate mesenchymal cells[J].China Journal of Oral and Maxillofacial Surgery,2010,8(2):171-177. |
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| Authors: | WANG Miao HUANG Hong-zhang HOU Jin-song |
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| Affiliation: | WANG Miao,HUANG Hong-zhang,HOU Jin-song.(Department of Oral , Maxillofacial Surgery,Guanghua College of Stomatology,Sun Yat-sen University.Guangzhou 510056,Guangdong Province,China) |
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| Abstract: | PURPOSE:To elucidate the mechanism by which all-trans retinoic acid(atRA) induces cleft palate.METHODS:The cell cycle distribution of mouse embryonic palate mesenchymal(MEPM) cells under atRA(100 mg/kg) treatment on gestation day(GD) 10 or GD 12 were measured by immunohistochemistry and flow cytometry.The p21 and phospho-Rb protein expression levels were detected by Western blot,respectively.Quantitative real-time PCR was performed for p21,pRb gene expression in each group under both conditions.The data wer... |
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| Keywords: | All-trans retinoic acid Embryonic palate mesenchymal cells p21 pRb Cell cycle |
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