粗化处理对新型骨植入钛合金的生物相容性影响

目的研究国内新研制的骨植入钛合金TZS进行喷砂并酸蚀处理对成骨细胞生物学行为的影响。方法采用SD大鼠体外原代培养方法培养成骨细胞，并将细胞分别接种于新型钛合金（ 光滑表面）及表面处理的材料表面，建立体外共同培养模型。采用MTT比色法检测第3天成骨细胞的增殖率，扫描电镜（ SEM）观察细胞形态学变化，培养第5天进行细胞碱性磷酸酶功能活性检测。结果喷砂与酸蚀处理组表面的成骨细胞，在3 d时的细胞增殖率与光滑组比较有统计学差异（ P<0.05）；5 d时的处理组碱性磷酸酶活性检测值与光滑组比较无统计学差异（ P>0.05）；SEM观察成骨细胞在喷砂与酸蚀组表面具有典型形态特征，伸展良好，具有丰富的板状、丝状伪足，优于光滑组。结论喷砂并酸蚀处理的新型钛合金在体外实验表现为不同程度的促进成骨细胞增殖及功能活性表达。

Studying the biocompatibility of implant titanium alloy after surface treatment by sandblasting and etching

Objective The aim of this study was to investigate the in vitro effect of cell behavior on a new titanium alloy and coarsening surface with sandblasting and duplicate treated by sandblasting and acid etching(SLA),including cell proliferation,morphology as well as alkaline phosphatase expression.Methods The cell derived from calvarial bone of neonate and co-cultured with the surface treated titanium alloys in vitro.The samples were treated by mechanical polishing,the sandblasting and the SLA.Cell proliferation on samples for 3 d was examined by MTT assay and cell morphology was observed by scan electron microscope.Alkaline phosphatase activity was measured at 5 d by ALP-special assay.Results The sandblasting and the SLA surface showed a significant different to smooth surface in the proliferation osteoblasts.The cells were elongated and spread well and having plentiful filopods on the coarse surface.The basal ALP activity indicated that optical density value of osteoblasts was not significant difference between coarse surface and smooth surface.Conclusion The observed effects are attributed to coarsening surface and support the concept that roughness can enhance the differentiation,good morphology and alkaline phosphatase expression of the cells.These results suggest that the good biological performance evaluation of sandblasting and etching surface is merited.