人牙龈和牙周韧带成纤维细胞体外矿化能力的比较研究

目的：研究人牙龈和牙周韧带成纤维细胞体外矿化能力的差异。方法：用组织块法培养同一患者的牙龈和牙周韧带成纤维细胞，取第4-8代细胞用矿化培养液长期培养，倒置显微镜下观察矿化情况，von Kos-sa染色显示钙盐沉积，生化法测定各组碱性磷酸酶活性，扫描电镜及X线能谱法分析矿化结节中钙磷比例。结果：倒置显微镜下观察牙周韧带成纤维细胞可呈复层生长，形成肉眼可见的白色结节，von Kossa染色显示结节内有钙盐沉积，碱性磷酸酶活性测定表明随着矿化培养时间的延长牙周韧带成纤维细胞组ALP活性比牙龈成纤维细胞组增高明显，扫描电镜下表明结节处电子反射增强，X射线能谱分析显示钙化结节内的钙磷比例与矿化组织相似；牙龈成纤维细胞及对照组体外不能形成钙化结节。结论：人牙龈和牙周韧带成纤维细胞体外矿化能力不同。

A comparative study of human gingival fibroblasts and periodontal ligament cells in their mineral characteristics in vitro

Objective To compare the mineral characteristics of human gingival fibroblasts with periodontal ligament cells in vitro. Methods Human gingival fibroblasts and periodontal ligament cells of the same patients were mineralized cultured, observed under microscope. The mineral nodules were dyed with von Kossa method. Alkaline phosphatase (ALP) activity was measured biochemically. The ratio of Ca and P was analyzed with energy dispersive X - ray analysis system. Results The results showed that it was periodontal ligament cells,not gingival fibroblasts, that could form mineral module routinely , von Kossa stain was positive, alkaline phosphatase activity was higher in periodontal linament cells than that in gingival fibroblasts. Prominent energy peaks which stand for calcium and phosphorumwere seen with energy dispersive X-ray microanalysis. Conclusion There is significant difference between gingival fibroblasts and periodontal ligament cells in the view of their mineral characteristic.