纳米磷酸三钙复合人工骨的Ames致突变试验

目的 从基因角度探讨纳米磷酸三钙／胶原复合人工骨的遗传毒理学特性。以了解此复合人工骨的生物相容性，为其在骨缺损修复领域的临床应用提供依据。方法 将纳米级磷酸三钙（β－TCP）同胶原复合，制成纳米β－TCP／胶原复合人工骨，并制备浸提液，采用Ames致突变试验，以生理盐水为阴性对照，测试其对鼠伤寒沙门菌的致突变比值（MR＝实验组回变菌落数Rt／阴性对照组回变菌落数Rc）。结果 阳性对照组各菌株回变菌落平均数均超过其相应阴性对照组回变菌落平均数的二倍以上（MRp＞2)，此复合人工骨的浸提液各剂量组对鼠伤寒沙门菌的致突变比值MR均小于2。结论 纳米磷酸三钙／胶原复合人工骨不会引起鼠伤寒沙门菌的回复突变数增加，提示此人工骨不会引起基因突变。

Ames test of nano-β-tricalcium phosphate /collagen artificial bone

Objective To study the inherited toxicology of nano-tricalcium phosphate/collagen (N-TCP/Co) artificial bone from the aspects of gene as to study the bio-compatibility and safety of it for the clinical use of bone's reconstruction.Methods Compound the N-TCP and Collagen to produce the N-TCP/Co artificial bone and it's diffusion. Test the diffusion through Ames test. The nature saline is the control. The markers of observation are the mutagenesis rates of murine typhoid salmonella (MR). Results The mutagenesis rates of murine typhoid salmonella in the experimental groups of all dosage levers are less than 2 while the MR of the positive control groups are more than 2.Conclusion The nano-tricalcium phosphate/collagen (N-TCP/Co) artificial bone will not cause the increase of the back mutation in murine typhoid salmonella which show that this artificial bone will not cause gene mutation.