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纳米羟基磷灰石复合骨髓基质细胞修复兔股骨缺损的实验研究
引用本文:谢红军,王银龙,周健,薛绍礼,何志良,解超勇,曹发明.纳米羟基磷灰石复合骨髓基质细胞修复兔股骨缺损的实验研究[J].口腔医学,2008,28(3):154-156.
作者姓名:谢红军  王银龙  周健  薛绍礼  何志良  解超勇  曹发明
作者单位:1. 安徽医科大学附属口腔医院口腔颌面外科,安徽,230032
2. 安徽医科大学生物工程教研室,安徽,230032
摘    要:目的探讨使用纳米羟基磷灰石(Nano-Hydroxyapitite,n-HA)作为骨组织工程支架材料的可行性。方法以成骨条件培养液培养兔骨髓基质细胞(BMSCs)至第3代并以1×106/cm2的密度与n-HA复合培养后回植于骨缺损区(1.0cm×0.8cm×0.5cm)。术后6周、12周处死动物,取缺损区及周围骨质进行肉眼、X线片、免疫组化染色观察及计算机图像分析。结果所有植入物无排除或感染。6周及12周通过肉眼及免疫组化观察实验组修复效果明显优于对照组,新骨面积百分比高于对照组(P<0.05),差异有统计学意义。结论n-HA作为骨组织工程支架材料具有可行性。

关 键 词:骨髓基质细胞  羟基磷灰石  成骨细胞  骨缺损  组织工程  
文章编号:1003-9872(2008)03-0154-03
修稿时间:2007年6月15日

Experiment of Nano-Hydroxyapitite bioceramics and bone marrow stromal cells to restore bone defect
XIE Hong-jun,WANG Yin-long,ZHON Jian,XUE Shao-li,HE Zhi-liang,XIE Chao-yong,CAO Fa-ming.Experiment of Nano-Hydroxyapitite bioceramics and bone marrow stromal cells to restore bone defect[J].Stomatology,2008,28(3):154-156.
Authors:XIE Hong-jun  WANG Yin-long  ZHON Jian  XUE Shao-li  HE Zhi-liang  XIE Chao-yong  CAO Fa-ming
Abstract:Objective To observe the feasibility of Nano-Hydroxyapitite(n-HA)as tissue engineering bone material.Methods BMSCs from the adult rabbit(n=6)marrow were cultured in vitro and induced to be osteoblast.The third generation BMSCs were combined with the n-HA and then implanted into the autologous furcation bone defects(1.0 cm×0.8 cm×0.85 cm).At 6 or 12 weeks,the rabbits were sacrificed.The specimens were processed for gross inspection,computer image analysis and immunohistochemical observation,respectively.Results The results of the experiment group were better than those of the control group,and the trabeculation of bone in the experiment group was earlier than that of the control group.The area in new bone in BMSCs-n-HA group was obviously higher than that of control group(P<0.05).Conclusion It is practicable to use Nano-Hydroxyapitite as tissue engineering bone material.
Keywords:bone marrow stromal cells  Hydroxyapitite  osteoblast  bone density  tissue engineering  rabbits
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